Effects of exponential feeding strategy on benzaldehyde lyase production by recombinant Escherichia coli

Effects of pretreated beet molasses on benzaldehyde lyase production by recombinant Escherichia coli BL21(DE3)pLySs Çalık, Pınar (Wiley, 2009-11-01) Aims:
Effects of pulse feeding of beet molasses on recombinant benzaldehyde lyase production by Escherichia coli BL21(DE3) Çalık, Pınar (Springer Science and Business Media LLC, 2009-11-01) The effect of fed-batch operation (FBO) strategy was investigated using pretreated-beet molasses, containing galactose that induces the lac promoter, on benzaldehyde lyase (BAL) production by recombinant Escherichia coli BL21(DE3)pLySs. After batch cultivation with 30 g l(-1) pretreated-beet molasses consisting of 7.5 g l(-1) glucose and 7.5 g l(-1) fructose, three FBO strategies were applied at dissolved oxygen (=40%) cascade to air-flow rate. In FBO1 when air-flow rate decreased considerably, feed was giv...
Effect of fed batch operation on intracellular reaction network of beta-lactamase producing Bacillus licheniformis Çalık, Pınar (2007-09-01)
Influence of controlled-pH and uncontrolled-pH operations on recombinant benzaldehyde lyase production by Escherichia coli Çalık, Pınar; Demir, AS (Elsevier BV, 2006-03-02) To select the host microorganism having the highest benzaldehyde lyase (BAL) production capacity, pUC 18::bal gene was transferred into four Escherichia coli strains. As the highest enzyme activity was obtained with E. coli K12 (ATCC 10798) carrying pUC18::bal gene, BAL production medium was designed for K 12. Using the designed medium containing 8.0 kg m(-3) glucose, 5.0 kg m(-3) (NH4)(2)HPO4 and the salt solution, the effects of uncontrolled-pH and controlled-pH operations were investigated at uncontrolle...
Role of the cmcH-ccaR intergenic region and ccaR overexpression in cephamycin C biosynthesis in Streptomyces clavuligerus Kurt, Aslihan; Alvarez-Alvarez, Ruben; Liras, Paloma; Özcengiz, Gülay (Springer Science and Business Media LLC, 2013-07-01) The effect of the CcaR regulatory protein on expression of the cephamycin C gene cluster is studied. Quantitative reverse transcription PCR (qRT-PCR) expression analysis of the cephamycin biosynthesis genes in the ccaR-disrupted strain, S. clavuligerus ccaR::aph, revealed that in the absence of CcaR, the lat and cmcI genes expression was reduced 2,200-and 1,087-fold compared with the wild type. Expression of pcbAB-pcbC-cefD-cefE-cmcJ-cmcH and blp was 225- to 359-fold lower, while expression of pcbR-pbpA-bla...

Citation Formats

H. Taspinar and P. Çalık, “Effects of exponential feeding strategy on benzaldehyde lyase production by recombinant Escherichia coli,” 2010, vol. 150, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/39610.