Alcohol determination via covalent enzyme immobilization on magnetic beads

Date

2008-01-15

Author

Kiralp, Senem
Topcu, Asuman
Bayramoglu, Guelay
Arica, M. Yakup
Toppare, Levent Kamil

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Alcohol oxidase was covalently immobilized onto magnetic beads of two different sizes, 75-150 mu m and 50-75 mu m in diameter, fabricated in the presence of glycidylmethacrylate and methylmethacrylate via suspension polymerization in the presence of a cross-linker (i.e., ethylenedimethylmethacrylate). The activity of the enzyme on smaller microspheres was found to be almost 4.8 fold higher than that of the larger counterparts. Although enzyme loading was same for both fractions, activity and the affinity of immobilized enzyme were significantly altered. The effects of various parameters such as temperature, pH, operational and storage stability were examined. The substantial change in the activity of enzyme was also observed in stability experiments in favor of large size magnetic beads. For all stability experiments including storage stability, the 75-150 mu m fraction was found to be more sentinel support for the enzyme.

Subject Keywords

Instrumentation, Electrical and Electronic Engineering, Materials Chemistry, Electronic, Optical and Magnetic Materials, Surfaces, Coatings and Films, Condensed Matter Physics, Metals and Alloys

URI

https://hdl.handle.net/11511/47582

Journal

SENSORS AND ACTUATORS B-CHEMICAL

DOI

https://doi.org/10.1016/j.snb.2007.07.035

Collections

Department of Chemistry, Article

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Citation Formats

S. Kiralp, A. Topcu, G. Bayramoglu, M. Y. Arica, and L. K. Toppare, “Alcohol determination via covalent enzyme immobilization on magnetic beads,” SENSORS AND ACTUATORS B-CHEMICAL, pp. 521–528, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/47582.