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Conducting polymers with benzothiadiazole and benzoselenadiazole units for biosensor applications
Date
2011-11-15
Author
Emre, Fatma Bilge
Ekiz, Fulya
Balan, Abidin
Emre, Sinan
TİMUR, SUNA
Toppare, Levent Kamil
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Poly(4,7-di(2,3)-dihydrothienol[3,4-b][1,4]dioxin-5-yl-benzo[1,2,5]thiadiazole) (PBDT) and poly(4,7-di(2,3)-dihydrothienol[3,4-b][1,4]dioxin-5-yl-2,1,3-benzoselenadiazole) (PESeE) were electrochemically deposited on graphite electrodes and used as immobilization matrices for biosensing studies. After electrochemical deposition of the polymeric matrices, glucose oxidase (GOx) was immobilized on the modified electrodes as the model enzyme. In the biosensing studies, the decrease in oxygen level as a result of enzymatic reaction was monitored at -0.7 V vs Ag/AgCl (3.0 M KCl) and correlated with substrate concentration. The biosensor was characterized in terms of several parameters such as operational and storage stabilities, kinetic parameters (K(m) and I(max)) and surface morphologies. The biosensor was tested on real human blood serum samples.
Subject Keywords
Instrumentation
,
Electrical and Electronic Engineering
,
Materials Chemistry
,
Electronic, Optical and Magnetic Materials
,
Surfaces, Coatings and Films
,
Condensed Matter Physics
,
Metals and Alloys
URI
https://hdl.handle.net/11511/34334
Journal
SENSORS AND ACTUATORS B-CHEMICAL
DOI
https://doi.org/10.1016/j.snb.2011.05.052
Collections
Department of Chemistry, Article