Immobilization of urease in conducting thiophene-capped poly(methyl methacrylate)/pyrrole matrices

Alkan, S
Toppare, Levent Kamil
Bakir, U
Yagci, Y
Urease was immobilized in conducting polypyrrole and block copolymers of thiophene-capped poly(methyl methacrylate) matrices by electropolymerization. Immobilization of the enzyme was achieved by application of 1.0 V constant potential in a solution of 0.02 M pyrrole, 2 mg/ml urease, 0.5 mg/ml supporting electrolyte. The optimum immobilization conditions, electrolysis time, electrolysis medium, enzyme concentration during electrolysis, type of supporting electrolyte were determined. The kinetic behaviors of free and immobilized urease were determined.


VOIGT, M; HALLENSLEBEN, ML; Toppare, Levent Kamil (Elsevier BV, 1993-03-22)
Several monosubstituted 3-arylazopyrrole derivatives were synthesized for the first time. The oxidation potentials were correlated to the Hammett constants of the substituents. Cyclic voltammetry studies revealed several interesting, features concerning the new compounds. Electrochemical homopolymerizations as well as copolymerization with pyrrole were performed.
Synthesis and characterization of conducting copolymers of succinic acid bis-(4-pyrrol-1-yl-phenyl) ester and their electrochromic properties
Ertas, M; Çırpan, Ali; Toppare, Levent Kamil (Elsevier BV, 2004-05-07)
A new dipyrrolyl monomer was synthesized via the reaction between 4-pyrrol-1-yl phenol and butanedioyl dichloride. Electrochemical behavior of this monomer, succinic acid bis-(4-pyrrol-1-yl-phenyl) ester (SM) was studied. Homopolymerization was achieved by chemical and constant Current electrolysis methods. Copolymerization of SM with thiophene was performed by constant potential electrolysis in acetonitrile (AN)-tetrabutylammonium tetrafluoroborate (TBAFB) and dichloromethane (DCM)-TBAFB, solvent-electroly...
Synthesis and characterization of graft copolymers of poly(2-(N-pyrrolyl)ethylvinylether) and polypyrrole
Kalaycioglu, E; Toppare, Levent Kamil; Yagci, Y (Elsevier BV, 2000-01-03)
Poly(2-(N-pyrrolyl)ethylvinylether) (poly(2-NEVE)) was synthesized from poly(2-chloroethylvinylether) (poly(2-CEVE)) via phase catalysis reaction. Graft copolymers of PPy/poly(2-NEVE) were synthesized by electrochemical methods. The chemical structure of poly(2-NEVE) was investigated by several spectroscopic and thermal methods. Graft copolymers were characterized by means of FTIR, Differential Scanning Calorimetry (DSC), Thermal Gravimetry Analysis (TGA), Scanning Electron Microscope (SEM). Conductivities ...
GEISSLER, U; HALLENSLEBEN, ML; Toppare, Levent Kamil (Elsevier BV, 1993-03-22)
Several vinylcarbazole derivatives were electrochemically polymerized into electrochromic films with electrical conductivities in the range of 10(-4) to 10(-7) S/cm. The characteristic white/green color change upon doping was due to bicarbazolyl groups. The cyclic voltammograms of the monomers revealed small differences due to the various positions of the vinyl group on the ring.
Lattice dynamical and thermo-elastic properties of M2AlB (M = V, Nb, Ta) MAX phase borides
Surucu, Gokhan; Gencer, Ayşenur; Wang, Xiaotian; Surucu, Ozge (Elsevier BV, 2020-04-05)
The structural, electronic, dynamic, and thermo-elastic properties of M2AlB (X = V, Nb, Ta) MAX phase borides were investigated using first principle calculations as implemented in the Vienna Ab-initio Simulation Package (VASP) with the generalized gradient approximation (GGA). The obtained structural properties and formation energies showed the thermodynamic stability and synthesizability of M2AlB. The electronic band structures were determined and they revealed that these compounds had a metallic characte...
Citation Formats
S. Alkan, L. K. Toppare, U. Bakir, and Y. Yagci, “Immobilization of urease in conducting thiophene-capped poly(methyl methacrylate)/pyrrole matrices,” SYNTHETIC METALS, pp. 95–99, 2001, Accessed: 00, 2020. [Online]. Available: