Immobilization of urease in conducting thiophene-capped poly(methyl methacrylate)/pyrrole matrices

Date

2001-08-22

Author

Alkan, S
Toppare, Levent Kamil
Bakir, U
Yagci, Y

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Urease was immobilized in conducting polypyrrole and block copolymers of thiophene-capped poly(methyl methacrylate) matrices by electropolymerization. Immobilization of the enzyme was achieved by application of 1.0 V constant potential in a solution of 0.02 M pyrrole, 2 mg/ml urease, 0.5 mg/ml supporting electrolyte. The optimum immobilization conditions, electrolysis time, electrolysis medium, enzyme concentration during electrolysis, type of supporting electrolyte were determined. The kinetic behaviors of free and immobilized urease were determined.

Subject Keywords

Mechanical Engineering, Materials Chemistry, Mechanics of Materials, Electronic, Optical and Magnetic Materials, Condensed Matter Physics, Metals and Alloys

URI

https://hdl.handle.net/11511/48592

Journal

SYNTHETIC METALS

DOI

https://doi.org/10.1016/s0379-6779(00)00580-4

Collections

Department of Chemistry, Article

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POLY(3-PHENYL AZOPYRROLE)S - A NEW CLASS OF CONDUCTING POLYMERS IN THE POLY(PYRROLE) SERIES VOIGT, M; HALLENSLEBEN, ML; Toppare, Levent Kamil (Elsevier BV, 1993-03-22) Several monosubstituted 3-arylazopyrrole derivatives were synthesized for the first time. The oxidation potentials were correlated to the Hammett constants of the substituents. Cyclic voltammetry studies revealed several interesting, features concerning the new compounds. Electrochemical homopolymerizations as well as copolymerization with pyrrole were performed.
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Citation Formats

S. Alkan, L. K. Toppare, U. Bakir, and Y. Yagci, “Immobilization of urease in conducting thiophene-capped poly(methyl methacrylate)/pyrrole matrices,” SYNTHETIC METALS, pp. 95–99, 2001, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48592.