Robust Covalent Coupling Scheme for the Development of FRET Aptasensor based on Amino-Silane-Modified Graphene Oxide

Date

2018-12-04

Author

Kahyaoğlu, Leyla Nesrin

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

225
views

0
downloads

In recent years, numerous aptamers have been physisorbed on graphene oxide (GO) to develop fluorescence resonance energy transfer-based aptasensors using the fluorescence quenching property of GO. However, physisorbed aptasensors show poor signal reversibility and reproducibility as well as nonspecific probe displacement, and thereby are not suitable for many analytical applications. To overcome these problems when working with complex biological samples, we developed a facile and robust covalent surface functionalization technique for GO-based fluorescent aptasensors using a well-studied adenosine triphosphate binding aptamer (ABA). In the scheme, GO is first modified with amino-silane, and further with glutaraldehyde to create available carbonyl groups for the covalent attachment of a fluorophore and an amino dual modified ABA. The surface modification method was characterized by zeta-potential, X-ray photoelectron spectroscopy, and Fourier-transform infrared spectroscopy (FTIR). The linearity, sensitivity, selectivity, and reversibility of the resulting GO-based covalent aptasensor was determined and systematically compared with the physisorbed aptasensor. Although both sensors showed similar performance in terms of sensitivity and linearity, better selectivity and higher resistance to nonspecific probe displacement was achieved with the developed covalent ABA sensor. The surface modification technique developed here is independent of the aptamer sequence, and therefore could be used universally for different analytical applications simply by changing the aptamer sequence for the target biomolecule.

Subject Keywords

Spectroscopy, Electrochemistry, General Materials Science, Surfaces and Interfaces, Condensed Matter Physics

URI

https://hdl.handle.net/11511/48674

Journal

LANGMUIR

DOI

https://doi.org/10.1021/acs.langmuir.8b02663

Collections

Department of Food Engineering, Article

Suggestions

OpenMETU
Core

Enhancement of H-2 Storage in Carbon Nanotubes via Doping with a Boron Nitride Ring Onay, Aytun Koyuncular; Erkoç, Şakir (American Scientific Publishers, 2009-04-01) Hydrogen storage capacity of carbon nanotubes with different chirality have been investigated by performing quantum chemical methods at semiempirical and DFT levels of calculations. It has been found that boron nitrite substitutional doping increases the hydrogen storage capacity of carbon nanotubes.
Amphoteric surface hydrogels derived from hydrogen-bonded multilayers: Reversible loading of dyes and macromolecules Kharlampieva, Eugenia; Erel Göktepe, İrem; Sukhishvili, Svetlana A. (American Chemical Society (ACS), 2007-01-02) We used hydrogen-bonded multilayers of poly(N-vinylpyrrolidone) (PVPON) and poly(methacrylic acid) (PMAA) as precursors for producing surface-bound hydrogels and studied their pH-dependent swelling and protein uptake behavior using in situ attenuated total reflection Fourier transform infrared spectroscopy and in situ ellipsometry. The hydrogels were produced by selective chemical cross-linking between PMAA units using carbodiimide chemistry and ethylenediamine (EDA) as a cross-linking reagent, followed by ...
A novel experimental and density functional theory study on palladium and nitrogen doped few layer graphene surface towards glucose adsorption and electrooxidation Caglar, Aykut; Duzenli, Derya; Önal, Işık; Tezsevin, Ilker; Sahin, Ozlem; Demir Kıvrak, Hilal (Elsevier BV, 2021-03-01) At present, few layer graphene (G) and nitrogen doped few layer graphene (N doped-G) are firstly coated on Cu foil via chemical vapor deposition (CVD) method and G and N doped-G coated Cu foil is transferred to the indium tin oxide (ITO) substrate surface to obtain electrodes. Pd metal is electrodeposited onto the N doped-G/ITO electrode (Pd-N doped-G/ITO). Pd-N doped-G/ITO electrode are characterized with advanced surface characterization methods such as Raman spectroscopy and SEM-EDX. Characterization res...
A comparative ab initio study of the ferroelectric behaviour in KNO3 and CaCO3 Aydınol, Mehmet Kadri; Alpay, S. P. (IOP Publishing, 2007-12-12) Potassium nitrate exhibits a reentrant phase transformation, where a metastable ferroelectric phase (gamma-KNO3) is formed upon cooling from high temperature. The layered structure of this ferroelectric phase is composed of alternating layers of potassium ions and nitrate groups; wherein, a central nitrogen atom is coordinated by three equilateral triangular oxygen atoms. The group layer is located less than midway between the cation layers, giving rise to a polar structure. From a structural perspective, t...
Design Parameters and Principles of Liquid-Crystal-Templated Synthesis of Polymeric Materials via Photolithography AKDENİZ, BURAK; Büküşoğlu, Emre (American Chemical Society (ACS), 2019-10-08) The design parameters and principles for the synthesis of polymeric microscopic objects using a method that combines photolithography and liquid crystal (LC) molecular templates have been demonstrated. Specifically, mixtures of a reactive mesogen (RM257) and nonreactive LC (E7) were polymerized using UV light and a photomask. We used photomasks with circular, triangular, rectangular, square, star-shaped, and heart-shaped features to provide initial shapes to the objects. Then, the unreacted parts were extra...

Citation Formats

L. N. Kahyaoğlu, “Robust Covalent Coupling Scheme for the Development of FRET Aptasensor based on Amino-Silane-Modified Graphene Oxide,” LANGMUIR, pp. 14586–14596, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48674.