Enhanced pectinase production by optimizing fermentation conditions of Bacillus subtilis growing on hazelnut shell hydrolyzate

Date

2015-03-01

Author

Uzuner, Sibel
Çekmecelioğlu, Deniz

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This study describes optimization of polygalacturonase (PG) production using Bacillus subtilis in submerged fermentation by Plackett-Burman (PB) design and response surface methodology (RSM). Five variables (pH, time, temperature, yeast extract concentration and K2HPO4), which were determined to be significant by the PB analysis, were further optimized using Box-Behnken response surface method. The optimization results indicated that a maximal PG activity of 5.60 U mL(-1) was achieved at pH 7.0, 72 h, and 30 degrees C using 0.5% (w/v) yeast extract and 0.02% (w/v) K2HPO4 in the fermentation medium. The results implied a 2.7-fold increase in PG activity of B. subtilis under the optimized conditions. Thus, it was concluded that hazelnut shell hydrolyzate have remarkable potential for low cost commercial PG production.

Subject Keywords

Hazelnut shell hydrolyzate, Pectinases, Plackett-burman design, Polygalacturonase activity, Response surface optimization

URI

https://hdl.handle.net/11511/49183

Journal

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

DOI

https://doi.org/10.1016/j.molcatb.2015.01.003

Collections

Department of Food Engineering, Article

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Citation Formats

S. Uzuner and D. Çekmecelioğlu, “Enhanced pectinase production by optimizing fermentation conditions of Bacillus subtilis growing on hazelnut shell hydrolyzate,” JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, pp. 62–67, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/49183.