Submerged cultivation of Scytalidium thermophilum on complex lignocellulosic biomass for endoglucanase production

Date

2001-05-07

Author

Ögel, Zümrüt Begüm
Yarangumeli, K
Dundar, H
Ifrij, I

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Scytalidium thermophilum endoglucanase production was analyzed on lignocellulosic biomass in submerged cultures at 45 degreesC and 155 rpm for S days. Endoglucanase, adsorbability of endoglucanase onto avicel, as well as exoglucanase, and filter paper activities were determined and compared with those on microcrystalline cellulose (avicel) as the main source of carbon. Lentil bran and sunflower seed bagasse yielded c. 1.5 fold more endoglucanase and avicel-adsorbable endoglucanase activity than avicel, and activities on grass clippings were similar. Grass clippings yielded the highest percentage of avicel-adsorbable endoglucanase among all lignocellulosic substrates tested. By the time when endoglucanase activities reached maximal levels, exo,glucanase activities on lentil bran, sunflower seed bagasse and grass clippings were c. 1.5-3 fold lower than those on avicel, although a significant difference in filter paper activities was not observed. On lignocellulosic biomass, maximum levels of endoglucanase activity were reached within 3-4 days, and within 6-7 days on avicel. (C) 2001 Elsevier Science Inc. All rights reserved.

Subject Keywords

Biotechnology, Applied Microbiology and Biotechnology, Biochemistry

URI

https://hdl.handle.net/11511/51500

Journal

ENZYME AND MICROBIAL TECHNOLOGY

DOI

https://doi.org/10.1016/s0141-0229(01)00315-5

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

Z. B. Ögel, K. Yarangumeli, H. Dundar, and I. Ifrij, “Submerged cultivation of Scytalidium thermophilum on complex lignocellulosic biomass for endoglucanase production,” ENZYME AND MICROBIAL TECHNOLOGY, pp. 689–695, 2001, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/51500.