Detection of physically interacting proteins with the CC and NB-ARC domains of a putative yellow rust resistance protein, Yr10, in wheat

Yildirim-Ersoy, Figen
Ridout, Christopher J.
Akkaya, Mahinur
Gene-for-gene (GFG) resistance is a potent defense mechanism in plants, that is mediated by resistance (R) proteins. In GFG resistance, pathogen effector or avirulence (Avr) proteins are recognised by R-proteins which initiate a series of signal transduction events that lead to hypersensitive cell death. In cereals, many R-proteins are comprised of an N-terminal coiled-coiled (CC) domain, a Nucleotide Binding (NB) domain and a Leucine Rich Repeats (LRR) region associated with effector recognition. NB-LRR immunity proteins are highly conserved across plant and animal taxa. To advance understating of signal transduction events in cereals, we exploited the high level of protein conservation to first identify yeast gene products interacting with the CC and NB domains of the candidate yellow rust R-protein (Yr10). Screening revealed proteins having mostly apoptosis related functions (Signal Recognition Particle 72kDa (SRP72); Chromosome SEgregation 1 (CSE1); ARrest Defective 1 (ARD1), translation initiation control in response to stress conditions (General Control Nonderepressible 2 (GCN2)), and a HSP90 co-chaperone (Cyclosporin-sensitive proline rotamase 7 (CPR7)). We then identified the close homologues of the interactors in barley and show that they were induced at 6h and/or 12 h after infection in Mla3 mediated Powdery Mildew (Blumeria graminis f.sp. hordei, Bgh) disease resistance, suggesting their involvement in pathogen response.


Identification and characterization of hydrolytic enzymes from the midgut of Sunn Pest of wheat (Eurygaster integriceps)
Ogur, E.; YÜCEL, MUSTAFA; Öktem, Hüseyin Avni (Informa UK Limited, 2009-01-01)
To help in the development of Sunn Pest-resistant transgenic plants employing protease or alpha-amylase inhibitors, midgut hydrolytic enzymes of Sunn Pest (Eurygaster integriceps, Put.) (Heteroptera: Scutelleridae) were identified and characterized biochemically. We observed levels of very low proteolytic activity of trypsin (3 nmoles/min/mg), elastase (0.66 nmoles/min/mg) and leucine aminopeptidase-like (14.4 nmoles/min/mg) proteases, but no chymotrypsin and papain-like activity. Proteolytic activities wer...
Molecular and Elemental Characterization of Selected Turkish Durum Wheat Varieties
HAKKI, ERDOĞAN EŞREF; Dograr, Nurdan; Pandey, Anamika; Khan, Mohd. Kamran; HAMURCU, MEHMET; KAYIŞ, SEYİT ALİ; GEZGİN, SAİT; Olmez, Fatih; Akkaya, Mahinur (AcademicPres (EAP) Publishing House, 2014-07-01)
Combination of elemental and protein studies along with molecular data using microsatellite markers may lead CO the better and realistic determination of relatedness between the varieties and their populations. In this study, the extent of diversity among five Turkish durum wheat cultivars and their populations has been assessed using seven microsatellite markers and the elemental analysis together with the differences in their protein content. In molecular analysis, total 23 alleles have been obtained amon...
An effector ofPuccinia striiformisf. sp.triticitargets chloroplasts with a novel and robust targeting signal
Andac, Ayse; Ozketen, Ahmet Caglar; Dagvadorj, Bayantes; Akkaya, Mahinur (Springer Science and Business Media LLC, 2020-08-01)
Fungal pathogens secrete effector molecules into host plant cells to suppress host immunity to colonize plants. Ongoing efforts are being made to identify and characterize effector proteins in many fungal plant pathogens. Nevertheless, the precise biological and biochemical functions of many effectors, such as their trafficking from the pathogen to the host, have yet to be fully understood. In this study, we show that an effector candidate, matured PstCTE1 ofPuccinia striiformisf. sp.tritici, localizes to c...
WANG, WC; SHANG, XM; YUCEL, M; NGUYEN, HT (Wiley, 1993-03-01)
In vitro culture offers approaches to study cellular responses and to obtain cells tolerant to high temperature stress. The objectives of this study were to select wheat cells that tolerate high temperature stress, study cell line stability and cytogenetic characteristics, and compare protein synthesis in selected and control cell lines after exposure to heat stress. Three of 600 colonies plated were recovered after cells had been exposed to 48-degrees-C. Forty-five percent of the cells of the tolerant cell...
Detection of contaminated hazelnuts and ground red chili pepper flakes by multispectral imaging
Kalkan, H.; Beriat, P.; Yardimci, Y.; Pearson, T. C. (Elsevier BV, 2011-06-01)
Mycotoxins are the toxic metabolites of certain filamentous fungi and have been demonstrated to cause various health problems in humans, including immunosuppression and cancer. Among them, the aflatoxins have received greater attention because they are potent carcinogens and are responsible for many human deaths per annum, mostly in non-industrialized countries. Various regulatory agencies have enforced limits on the concentrations of these toxins in foods and feeds involved in international commerce. Hyper...
Citation Formats
F. Yildirim-Ersoy, C. J. Ridout, and M. Akkaya, “Detection of physically interacting proteins with the CC and NB-ARC domains of a putative yellow rust resistance protein, Yr10, in wheat,” JOURNAL OF PLANT DISEASES AND PROTECTION, pp. 119–126, 2011, Accessed: 00, 2020. [Online]. Available: