An effector ofPuccinia striiformisf. sp.triticitargets chloroplasts with a novel and robust targeting signal

2020-08-01
Andac, Ayse
Ozketen, Ahmet Caglar
Dagvadorj, Bayantes
Akkaya, Mahinur
Show full item record
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
228
views
0
downloads
Fungal pathogens secrete effector molecules into host plant cells to suppress host immunity to colonize plants. Ongoing efforts are being made to identify and characterize effector proteins in many fungal plant pathogens. Nevertheless, the precise biological and biochemical functions of many effectors, such as their trafficking from the pathogen to the host, have yet to be fully understood. In this study, we show that an effector candidate, matured PstCTE1 ofPuccinia striiformisf. sp.tritici, localizes to chloroplasts when expressed inplanta. It has no conserved transit signal region that can be detected by widely accepted prediction tools including TargetP and ChloroP, it must be carrying a unique localization signal. We have shown that N-terminal tagged red fluorescent protein has no effect on the chloroplast localization of PstCTE1, suggesting a new chloroplast translocation mechanism. We also observed the entrance of the candidate effector to the chloroplast even with the construct having the intact signal peptide on the N-terminus of the transit peptide region. Possibly due to overexpression of the protein inN. benthamina, accumulation in the ER (cytoplasm) was obvious. As previously reported, PstCTE1, similar to effector proteins, may either escape from the secretory pathway by retrograde transport, or translation may occur at alternative sites. This would result in a truncated and/or non-functional signal peptide at the N-terminus in a non-host model system (Nicotiana benthamiana), if it is not re-entering the cell from the apoplast. Our study adds PstCTE1 to the pool of few candidate effectors, experimentally shown to target the chloroplast.
Agronomy and Crop Science, Plant Science, Horticulture
https://hdl.handle.net/11511/56966
EUROPEAN JOURNAL OF PLANT PATHOLOGY
Graduate School of Natural and Applied Sciences, Article

Suggestions

Detection of physically interacting proteins with the CC and NB-ARC domains of a putative yellow rust resistance protein, Yr10, in wheat
Yildirim-Ersoy, Figen; Ridout, Christopher J.; Akkaya, Mahinur (Springer Science and Business Media LLC, 2011-08-01)
Gene-for-gene (GFG) resistance is a potent defense mechanism in plants, that is mediated by resistance (R) proteins. In GFG resistance, pathogen effector or avirulence (Avr) proteins are recognised by R-proteins which initiate a series of signal transduction events that lead to hypersensitive cell death. In cereals, many R-proteins are comprised of an N-terminal coiled-coiled (CC) domain, a Nucleotide Binding (NB) domain and a Leucine Rich Repeats (LRR) region associated with effector recognition. NB-LRR im...
SELECTION OF CULTURED WHEAT CELLS FOR TOLERANCE TO HIGH-TEMPERATURE STRESS
WANG, WC; SHANG, XM; YUCEL, M; NGUYEN, HT (Wiley, 1993-03-01)
In vitro culture offers approaches to study cellular responses and to obtain cells tolerant to high temperature stress. The objectives of this study were to select wheat cells that tolerate high temperature stress, study cell line stability and cytogenetic characteristics, and compare protein synthesis in selected and control cell lines after exposure to heat stress. Three of 600 colonies plated were recovered after cells had been exposed to 48-degrees-C. Forty-five percent of the cells of the tolerant cell...
Detection of contaminated hazelnuts and ground red chili pepper flakes by multispectral imaging
Kalkan, H.; Beriat, P.; Yardimci, Y.; Pearson, T. C. (Elsevier BV, 2011-06-01)
Mycotoxins are the toxic metabolites of certain filamentous fungi and have been demonstrated to cause various health problems in humans, including immunosuppression and cancer. Among them, the aflatoxins have received greater attention because they are potent carcinogens and are responsible for many human deaths per annum, mostly in non-industrialized countries. Various regulatory agencies have enforced limits on the concentrations of these toxins in foods and feeds involved in international commerce. Hyper...
Molecular and Elemental Characterization of Selected Turkish Durum Wheat Varieties
HAKKI, ERDOĞAN EŞREF; Dograr, Nurdan; Pandey, Anamika; Khan, Mohd. Kamran; HAMURCU, MEHMET; KAYIŞ, SEYİT ALİ; GEZGİN, SAİT; Olmez, Fatih; Akkaya, Mahinur (AcademicPres (EAP) Publishing House, 2014-07-01)
Combination of elemental and protein studies along with molecular data using microsatellite markers may lead CO the better and realistic determination of relatedness between the varieties and their populations. In this study, the extent of diversity among five Turkish durum wheat cultivars and their populations has been assessed using seven microsatellite markers and the elemental analysis together with the differences in their protein content. In molecular analysis, total 23 alleles have been obtained amon...
Applicability of optimised in-vessel food waste composting for windrow systems
Çekmecelioğlu, Deniz; Graves, RE; Davitt, NH (Elsevier BV, 2005-08-01)
The presence of pathogens in various organic wastes requires careful attention to the composting process to minimise the chance of their survival. An optimised composting mixture from previous in-vessel composting studies (50% food waste, 40% manure, and 10% bulking agent) was evaluated using windrow composting. Conventional layering and mixing methods were used for constructing the windrows. Temperature was monitored as the indication of the efficiency of composting. Initial and final physico-chemical char...
Citation Formats
A. Andac, A. C. Ozketen, B. Dagvadorj, and M. Akkaya, “An effector ofPuccinia striiformisf. sp.triticitargets chloroplasts with a novel and robust targeting signal,” EUROPEAN JOURNAL OF PLANT PATHOLOGY, pp. 751–765, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/56966.
METU IIS - Integrated Information Service open@metu.edu.tr