Amifostine, a radioprotectant agent, protects rat brain tissue lipids against ionizing radiation induced damage: An FTIR microspectroscopic imaging study

Date

2012-04-15

Author

Cakmak, Gulgun
Miller, Lisa M.
Zorlu, Faruk
Severcan, Feride

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

153
views

0
downloads

Amifostine is the only approved radioprotective agent by FDA for reducing the damaging effects of radiation on healthy tissues. In this study, the protective effect of amifostine against the damaging effects of ionizing radiation on the white matter (WM) and grey matter (GM) regions of the rat brain were investigated at molecular level. Sprague-Dawley rats, which were administered amifostine or not, were whole-body irradiated at a single dose of 800 cGy, decapitated after 24 h and the brain tissues of these rats were analyzed using Fourier transform infrared microspectroscopy (FTIRM). The results revealed that the total lipid content and CH2 groups of lipids decreased significantly and the carbonyl esters, olefinic=CH and CH3 groups of lipids increased significantly in the WM and GM after exposure to ionizing radiation, which could be interpreted as a result of lipid peroxidation. These changes were more prominent in the WM of the brain. The administration of amifostine before ionizing radiation inhibited the radiation-induced lipid peroxidation in the brain. In addition, this study indicated that FTIRM provides a novel approach for monitoring ionizing radiation induced-lipid peroxidation and obtaining different molecular ratio images can be used as biomarkers to detect lipid peroxidation in biological systems.

Subject Keywords

Biophysics, Biochemistry, Molecular Biology

URI

https://hdl.handle.net/11511/52463

Journal

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

DOI

https://doi.org/10.1016/j.abb.2012.02.012

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

BIOCHEMICAL ASPECTS OF FREE-RADICALS BASAGA, HS (Canadian Science Publishing, 1990-07-01) Toxic free radicals can be produced by many reactions required for the maintenance of normal metabolism and the production of energy in the cell. The reactivity of both primary and secondary radicals with biomolecules and in whole tissue systems is of interest, not only because of their importance in radiobiology but also because of the role these species play in toxicity and various disorders. Oxidant stress is known to increase the production of free radicals. In the presence of metals, especially iron, t...
Pathogen detection by core-shell type aptamer-magnetic preconcentration coupled to real-time PCR Ozalp, V. Cengiz; BAYRAMOĞLU, GÜLAY; Kavruk, Murat; Keskin, Batuhan B.; Öktem, Hüseyin Avni; ARICA, MEHMET YAKUP (Elsevier BV, 2014-02-15) The presence of pathogenic bacteria is a major health risk factor in food samples and the commercial food supply chain is susceptible to bacterial contamination. Thus, rapid and sensitive identification methods are in demand for the food industry. Quantitative polymerase chain reaction (PCR) is one of the reliable specific methods with reasonably fast assay times. However, many constituents in food samples interfere with PCR, resulting in false results and thus hindering the usability of the method. Therefo...
Modulation of human flavin-containing monooxygenase 3 activity by tricyclic antidepressants and other agents: Importance of residue 428 Adalı, Orhan; Philpot, RM (Elsevier BV, 1998-10-01) Human flavin-containing monooxygenase 3 (FMO3) is subject to modulation by tricyclic antidepressants and other agents. Imipramine activates FMO3-catalyzed metabolism of methimazole at all substrate concentrations tested. This distinguishes FMO3 from rabbit FMO1 and FMO2, which are activated at high substrate concentration and inhibited at low substrate concentration, and pig FMO1, which is inhibited at all substrate concentrations. The response of FMO3 is also unique in that chlorpromazine is markedly more ...
Evaluation of colloidal platinum on cytotoxicity, oxidative stress and barrier permeability across the gut epithelium Tuncer, Sinem; Colakoglu, Melis; Ulusan, Sinem; Ertaş, Gülay; KARASU, Çimen; Banerjee, Sreeparna (2019-03-01) Colloidal platinum (Pt) is widely consumed due to its health promoting benefits. However, the exact biological effects of these nanoparticles have not been studied in detail, particularly in the gut. In the present study we observed that colloidal Pt was not cytotoxic towards three different epithelial colon cancer cell lines. Co-treatment of the colon cancer cell line Caco-2 with the oxidative stress inducing agent hydrogen peroxide (H2O2) and colloidal Pt resulted in a significant decrease in H2O2 induced...
Gene expressions of Mn-SOD and GPx-1 in streptozotocin-induced diabetes: effect of antioxidants Sadi, Goekhan; Güray, Nülüfer Tülün (Springer Science and Business Media LLC, 2009-07-01) Increased oxidative stress and impaired antioxidant defense mechanisms are believed to be the important factors contributing to the pathogenesis and progression of diabetes mellitus. In this study, we have reported the effects of the streptozotocin-induced diabetes on the gene expression and the activities of two antioxidant enzymes, manganese superoxide dismutase (MnSOD) and glutathione peroxidase (GPx). We also studied the effects of two antioxidants, vitamin C and DL-alpha-lipoic acid (LA), on the system...

Citation Formats

G. Cakmak, L. M. Miller, F. Zorlu, and F. Severcan, “Amifostine, a radioprotectant agent, protects rat brain tissue lipids against ionizing radiation induced damage: An FTIR microspectroscopic imaging study,” ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, pp. 67–73, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/52463.