Crossflow ultrafiltration of Bacillus licheniformis fermentation medium to separate protease enzymes

Date

2001-01-01

Author

Takac, S
Elmas, S
Çalık, Pınar
Ozdamar, TH

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Separation conditions for the serine alkali protease (SAP) enzyme from the neutral protease and amylase enzymes of Bacillus licheniformis cells were investigated in a crossflow ultrafiltration system by using 30 000 Da MWCO polysulphone membrane. The effects of initial enzyme concentration and recirculation velocity on the permeate flux, on the total resistance, and on the recovery yield of SAP in the permeate were investigated. High permeate flux was obtained at high recirculation velocity but at low initial enzyme concentration, where the SAP enzyme activity was best recovered at low velocity and enzyme concentration conditions.

Subject Keywords

Proteins, UF, Siimulatiion, Adsorptiion, Pore-siize diistriibutiions, Flow ultrafiiltratiion, Membranes, Transport, Retentiion

URI

https://hdl.handle.net/11511/53083

Collections

Department of Chemical Engineering, Conference / Seminar