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IMMOBILIZATION OF NADH-CYTOCHROME B5 REDUCTASE INTO GELATIN BY CROSS-LINKING
Date
1994-01-01
Author
Yıldırım, Onur
Akbulut, Ural
Metadata
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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In this work NADH-cytochrome b5 reductase enzyme (EC 1.6.2.2) obtained from rabbit liver microsomes was immobilized into gelatin by crosslinking. Chromium (III) acetate and chromium (III) sulfate were used as crosslinkers. To find the most suitable immobilization parameters; effect of crosslinker, enzyme and gelatin concentrations on immobilized enzyme activity were investigated. Unbounded enzyme was determined by washing experiments.
Subject Keywords
Nadh-Cytochrome-B5 reductase
,
Liver-microsomes
,
Sheep lung
,
Purification
URI
https://hdl.handle.net/11511/54360
Journal
JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY
Collections
Department of Economics, Article
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O. Yıldırım and U. Akbulut, “IMMOBILIZATION OF NADH-CYTOCHROME B5 REDUCTASE INTO GELATIN BY CROSS-LINKING,”
JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY
, pp. 19–28, 1994, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/54360.