Immobilization of cholesterol oxidase in a conducting copolymer of thiophene-3-yl acetic acid cholesteryl ester with pyrrole

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2003-01-01

Author

Çırpan, Ali
Toppare, Levent Kamil
YAGCI, Y

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Cholesterol oxidase has been immobilized in conducting copolymers of thiophene-3-yl acetic acid cholesteryl ester with pyrrole (CM/PPy) and polypyrrole (PPy) via electropolymerization. p-Toluene sulphonic acid was used as the supporting electrolyte. Kinetic parameters (V-max and K-m) and operational stability of enzyme electrodes were investigated. Surface morphology of the films was examined by scanning electron microscope.

Subject Keywords

Materials Chemistry, General Chemistry, General Chemical Engineering, Polymers and Plastics

URI

https://hdl.handle.net/11511/34813

Journal

DESIGNED MONOMERS AND POLYMERS

DOI

https://doi.org/10.1163/156855503768336252

Collections

Department of Chemistry, Article

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Immobilization of Invertase in Copolymer of 2,5-Di(thiophen-2-yl)-1-p-Tolyl-1H-Pyrrole with Pyrrole Celebi, Selin; Ibibikcan, Esin; Kayahan, Senem; Yigitsoy, Basak; Toppare, Levent Kamil (Informa UK Limited, 2009-01-01) Immobilization of invertase in conducting copolymer matrix of 2,5-di(thiophen-2-yl)-1-p-tolyl-1H-pyrrole with pyrrole (poly(DDTP-co-Py)) was achieved via electrochemical polymerization. Kinetic parameters, Michaelis-Menten constant, Km and the maximum reaction rate, Vmax were investigated. Operational stability and temperature optimization of the enzyme electrodes were also examined. Immobilized invertase reveals maximum activity at 50 degrees C and; pH 8 and pH 4 for two copolymer matrices. Although the sa...

Citation Formats

A. Çırpan, L. K. Toppare, and Y. YAGCI, “Immobilization of cholesterol oxidase in a conducting copolymer of thiophene-3-yl acetic acid cholesteryl ester with pyrrole,” DESIGNED MONOMERS AND POLYMERS, pp. 237–243, 2003, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/34813.