Oxygen transfer regulates benzaldehyde lyase production in E-coli



Oxygen transfer effects on recombinant benzaldehyde lyase production
Çalık, Pınar; Ayhan, Peruze; Demir, Ayhan S. (Elsevier BV, 2004-11)
Oxygen transfer (OT) effects on benzaldehyde lyase (BAL) production by recombinant Escherichia coli carrying pUC18::bal gene were investigated on a defined medium with glucose and (NH4)(2)HPO4 as the sole carbon and nitrogen sources respectively, in 3.0 dm(3) bioreactor systems at six different conditions with the parameters, air inlet rate of Q(O)/V-R = 0.5 vvm, and agitation rates of N = 250, 375, 500, 625, 750 min(-1) and at Q(O)/V-R = 0.7 vvm, N = 750 min(-1). Cell concentration (C-X), BAL volumetric an...
Oxygen transfer effects in beta-lactamase fermentation by Bacillus licheniformis in a glucose-based defined medium
Çalık, Pınar; Calik, G (Wiley, 2005-09-01)
The effects of oxygen transfer on the beta-lactamase production by Bacillus licheniformis were investigated in a glucose-based defined medium. The experiments were conducted in 3.0 dm(3) batch bioreactor systems at three different air inlet (Q(O)/V-R = 0.2, 0.5 and 1.0 vvm) and agitation rates (N = 250, 500 and 750 min(-1)). During the fermentation, the concentrations of the cell, glucose, by-products, ie organic and amino acids, oxygen transfer coefficients (K(L)alpha), yield coefficients, specific rates a...
Oxygen transfer as a tool for fine-tuning recombinant protein production by Pichia pastoris under glyceraldehyde-3-phosphate dehydrogenase promoter
Gunes, Hande; Çalık, Pınar (2016-07-01)
Effects of oxygen transfer on recombinant protein production by Pichia pastoris under glyceraldehyde- 3-phosphate dehydrogenase promoter were investigated. Recombinant glucose isomerase was chosen as the model protein. Two groups of oxygen transfer strategies were applied, one of which was based on constant oxygen transfer rate where aeration rate was Q(O)/V = 3 and 10 vvm, and agitation rate was N = 900 min(-1); while the other one was based on constant dissolved oxygen concentrations, C-DO = 5, 10, 15, 20...
Oxygen adsorption on Pt/TiO2 catalysts
Üner, Deniz; Ozen, I; Uner, M (Elsevier BV, 2003-09-30)
Oxygen adsorption overPt/TiO2 surfaces were investigated as a function of metal loading. Oxygen adsorption over pure TiO2 was molecular at all pressure ranges investigated. On the other hand, differential heats of adsorption measured by adsorption calorimetry indicated that oxygen adsorption was dissociative over Pt/TiO2 surfaces until the Pt surface was saturated with oxygen. The saturation coverage of atomic oxygen on Pt was determined as approximately one from adsorption calorimetry. The initial heat of ...
Oxygen-transfer strategy and its regulation effects in serine alkaline protease production by Bacillus licheniformis
Çalık, Pınar; Özdamar, Tunçer H. (2000-08-01)
The effects of oxygen transfer on the production and product distribution in serine alkaline protease (SAP) fermentation by Bacillus licheniformis and oxygen-transfer strategy in relation to the physiology of the bacilli were investigated on a defined medium with citric acid as sole carbon source in 3.5-dm(3) batch bioreactor systems. By forming a 3 x 3 matrix with the parameters air-inlet rates of Q(O)/V-R = 0.2, 0.5, 1.0 vvm, and agitation rates of N = 150, 500, 750 min(-1), the effects of oxygen transfer...
Citation Formats
P. Çalık, “Oxygen transfer regulates benzaldehyde lyase production in E-coli,” 2005, vol. 118, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/55155.