Oxygen transfer effects in beta-lactamase fermentation by Bacillus licheniformis in a glucose-based defined medium

Date

2005-09-01

Author

Çalık, Pınar
Calik, G

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

207
views

0
downloads

The effects of oxygen transfer on the beta-lactamase production by Bacillus licheniformis were investigated in a glucose-based defined medium. The experiments were conducted in 3.0 dm(3) batch bioreactor systems at three different air inlet (Q(O)/V-R = 0.2, 0.5 and 1.0 vvm) and agitation rates (N = 250, 500 and 750 min(-1)). During the fermentation, the concentrations of the cell, glucose, by-products, ie organic and amino acids, oxygen transfer coefficients (K(L)alpha), yield coefficients, specific rates and oxygen uptake rates (OUR) were determined, in addition to beta-lactamase activities. The highest beta-lactamase activity was obtained at Q(O)/V-R = 0.5 vvm and N = 500 min-1 and at Q(O)/V-R = 0.2 vvm and N = 500 min-1 conditions, as ca A = 90 U cm(-3). The highest cell concentration was obtained as C-X = 0.67 kg m(-3) at Q(O)/V-R = 0.5 vvm and N = 750 min(-1) and at Q(O)/V-R = 0.2 vvm and N = 750 min(-1) conditions. The values of K(L)a increased with increasing agitation and aeration rates and varied between 0.007 and 0.044 s(-1), and the OUR varied between 0.4 and 1.6 mol m(-3) s(-1). With increasing Q(O)/V-R and/or N, the Damkohler number (ie the oxygen transfer limitation) decreased owing to the increase in mass transfer coefficients (K(L)a). The highest instantaneous yield of cell on substrate (Y-X/S) and yield of cell on oxygen (Y-X/O) values were respectively obtained at 0.5 vvm and 500 min-1 conditions at t = 2 h as Y-X/S = 0.72 kg kg 1 and Y-X/O = 1.49 kg kg(-1). The highest instantaneous yield of substrate on oxygen (Y-S/O) was obtained at 0.5 vvm and 750 min(-1) conditions at t = 20 h as Y-S/O = 8.07 kg kg(-1). (c) 2005 Society of Chemical Industry.

Subject Keywords

Biotechnology, Fuel Technology, Inorganic Chemistry, Renewable Energy, Sustainability and the Environment, Organic Chemistry, Waste Management and Disposal, Pollution, General Chemical Engineering

URI

https://hdl.handle.net/11511/37664

Journal

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

DOI

https://doi.org/10.1002/jctb.1284

Collections

Department of Chemical Engineering, Article

Suggestions

OpenMETU
Core

Fermentation and oxygen transfer characteristics in serine alkaline protease production by recombinant Bacillus subtilis in molasses-based complex medium Calik, G; Pehlivan, N; Ozcelik, IS; Çalık, Pınar; Ozdamar, TH (Wiley, 2004-11-01) Serine alkaline protease (SAP) production in a complex medium based on physically pretreated molasses by recombinant Bacillus subtilis carrying pHV1431::subC gene is described. The effects of oxygen transfer were investigated in 3.5 dm(3) bioreactor systems with controls for agitation rate, dissolved oxygen, pH, temperature, and foam formation under two different agitation rates, ie N = 500 and 750 min(-1), and four different air flow rates, ie Q/V-R = 0.2, 0.5, 0.7, and 1.0 vvm, at a molasses concentration...
Metabolic reaction network of Pichia pastoris with glycosylation reactions: Flux analysis for erythropoietin production Eskitoros, Melda S.; Ata, Ozge; Çalık, Pınar (Wiley, 2014-11-01) BACKGROUND: Biochemical reaction network of Pichia pastoris was improved by including N-glycosylation pathway reactions to determine the intracellular reaction rates for glycosylated protein production.
KINETICS OF WINE SPOILAGE BY ACETIC-ACID BACTERIA KOSEBALABAN, F; OZILGEN, M (Wiley, 1992-01-01) Acid production in wines inoculated with acetic acid bacteria from vinegar was studied at 15-degrees-C, 20-degrees-C, 25-degrees-C and 30-degrees-C and simulated with kinetic models. Results indicated that major wine spoilage did not occur during the growth of the microorganisms, but during their extended presence at high concentrations during the stationary and death phases. Acetophilic species were observed to resist death, which is in agreement with the mechanism suggested by Kittelmann et al.9 for the d...
Oxygen transfer effects in serine alkaline protease fermentation by Bacillus licheniformis: Use of citric acid as the carbon source Çalık, Pınar (Elsevier BV, 1998-12-01) The effects of oxygen transfer on serine alkaline protease (SAP) production by Bacillus licheniformis on a defined medium with C-c = 9.0 kg m(-3) citric acid as sole carbon source were investigated in 3.5 dm(3) batch bioreactor systems. The concentrations of the product (SAP) and by-products, i.e., neutral protease, amylase, amino acids, and organic acids were determined in addition to SAP activities. Ar Q(o)/V = 1 vvm airflow rate, the effect of agitation rate on DO concentration, pH, product, and by-produ...
Analytical and Numerical Modeling of CO2 Sequestration in Deep Saline Aquifers Ozgur, E.; Gumrah, F. (Informa UK Limited, 2010-01-01) The analytical and numerical modeling of CO2 sequestration in deep saline aquifers having different rock and fluid properties was studied under diffusion and convection mechanisms. In a diffusion dominated system, an aquifer with 100 m thickness was saturated with CO2 after 10,000,000 years. It was much earlier in a convective dominant system. In the diffusion process, the dissolution of CO2 in aquifer increased with porosity increase; however, in a convection dominant process dissolution of CO2 in aquifer ...

Citation Formats

P. Çalık and G. Calik, “Oxygen transfer effects in beta-lactamase fermentation by Bacillus licheniformis in a glucose-based defined medium,” JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, pp. 1062–1071, 2005, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37664.