Controlled release of bioactive agents in gene therapy and tissue engineering

Even though the drugs are effective in the treatment of some diseases, they may be inefficient or incapable of solving the problem in some other diseases. It is known that some diseases have genetic causes and therefore the search for a therapy in these cases is intense. The solutions involving either direct application of a gene or its basic product, proteins, especially the growth factors, are oftencontemplated. Gene therapy is a novel approach to treating diseases based on modifying the expression of a person’s genes toward a therapeutic goal. While genes and proteins offer a great opportunity for the treatment of many diseases their administration require critical consideration of the structure and conditions. When the conventional methods of drug administration is adopted for the administration of genes and proteins the results are not successful. Genetic material and the proteins are too labile or unstable due to their fragile 3-D structures and this makes their administration to a medium for long periods in unprotected form impossible. For gene delivery in vivo, additional problems have to be overcome, including the anatomical size constraints, protection from nonspecific interactions with body fluids, extracellular matrix and nontarget cells.


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Conventional methods of chemotherapy requires novel therapy systems due to serious side effects and inefficiency of drug administration. In recent years many studies are carried out to improve drug delivery systems. Polymers are one of the most important elements for drug delivery research due to their versatility. By the discovery of dendritic polymers, drug delivery studies gained a new vision. Highly branched monodisperse structure, multiple sites of attachment, well-defined size and controllable physica...
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Citation Formats
D. Keskin and V. N. Hasırcı, “Controlled release of bioactive agents in gene therapy and tissue engineering,” TISSUE ENGINEERING, STEM CELLS AND GENE THERAPIES, pp. 279–296, 2003, Accessed: 00, 2020. [Online]. Available: