Drug Resistant MCF-7 Cell Lines Also Developed Cross-Resistance to Structurally Unrelated Anticancer Agents

Date

2009-03-01

Author

Iseri, Ozlem D.
Kars, Meltem D.
Eroglu, Seckin
Gündüz, Ufuk

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The cells developing resistance to an applied drug may also present cross-resistance to other anticancer drugs which are not applied. In this study, the development of cross-resistance in paclitaxel (MCF-7/Pac), docetaxel (MCF-7/Doc), vincristine (MCF-7/Vinc) and doxorubicin (MCF-7/Dox) resistant MCF-7 cells to selective anticancer drugs, tamoxifen and all trans-retinoic acid (ATRA) were investigated. Combined antiproliferative effects of these drugs in different combinations were also evaluated by checkerboard combination assay. MCF-7/Pac and MCF-7/Doc cells developed cross-resistance to vincristine (13- and 12-folds, respectively) and tamoxifen (3- and 2-folds, respectively). MCF-7/Dox cells developed cross-resistance to paclitaxel (109-fold), docetaxel (10-fold), tamoxifen (2-fold) and ATRA (3-fold). MCF-7/Vinc cells developed cross-resistance to paclitaxel (48-fold), doxorubicin (6-fold) and tamoxifen (2-fold). Combinations of paclitaxel and docetaxel with doxorubicin exerted synergic anti proliferative effect. Tamoxifen had synergic effect with doxorubicin and vincristine. ATRA had indifferent effect with paclitaxel, docetaxel and doxorubicin where it had antagonistic effect with vincristine. The data presented here may provide an insight to assess response of breast tumors to anticancer drug combinations.

Subject Keywords

Multiple drug resistence, MCF-7, Cross-resistance, Chemotherapy

URI

https://hdl.handle.net/11511/55299

Journal

UHOD-ULUSLARARASI HEMATOLOJI-ONKOLOJI DERGISI

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

O. D. Iseri, M. D. Kars, S. Eroglu, and U. Gündüz, “Drug Resistant MCF-7 Cell Lines Also Developed Cross-Resistance to Structurally Unrelated Anticancer Agents,” UHOD-ULUSLARARASI HEMATOLOJI-ONKOLOJI DERGISI, pp. 1–8, 2009, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/55299.