Qualitative detection of GM maize (Bt 11) in food and feed sold commercially in Turkey by PCR based methods

Gültekin, Güzin Candan
Yilmaz, Remziye
To regulate the presence of genetically modified organisms (GMOs) in crops, foods and food ingredients, the development of reliable and sensitive methods for their detection is necessary. In the present study, products derived from GM maize in the Turkish market were monitored using methods to detect the presence of CaMV 35S promoter, nos terminator and Bt 11 maize. The results demonstrated, for the first time, the presence of GM maize in Turkish food and feed products qualitatively.


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Cansiz, Sena; Özen, Can; Bayrac, Ceren; Bayrac, A. Tahir; Gül, Fatma; Kavruk, Murat; Yilmaz, Remziye; EYİDOĞAN, FÜSUN; Öktem, Hüseyin Avni (2012-09-01)
Over the last decade, array detection has been developed to qualitatively assess the presence of genetically modified organisms (GMOs). To date, DNA array systems have the highest capabilities as a result of GMOs analysis. We describe the construction of an array platform in the sandwich hybridization format for the detection of transgenic promoter of Cauliflower mosaic virus (CaMV; p35S). Sequence-specific signal development has been achieved by a sandwich complex composed of a surface immobilized capture ...
Development of a loop mediated isothermal amplification (LAMP) based detection platform for genetically modified organism (GMO) detection
Moğol, Ayça Nazlı; Öktem, Hüseyin Avni; Department of Biotechnology (2019)
Genetically modified organisms (GMOs) are being widely used worldwide. Every country has a different legislation regarding the allowed events and GMO levels. This creates the great need for GMO detection. In this study, LAMP assay was used for GMO detection owing to its high sensitivity with the genetically modified organisms; Bt11 maize, GT73 Roundup Ready canola, and transgenic Nicotiana tabacum, targeting the sequences most commonly used in GMO constructions; 35S promoter and Figwort mosaic virus (FMV) s...
Detection of genetically modified insect resistant tomato via polymerase chain reaction
Sönmezalp, C. Zeynep; Gültekin, Güzin Candan; Department of Biotechnology (2004)
Tomato, which is one of the most important component of human diet, has been genetically modified to develop some properties like delayed ripening and insect resistance. In order to give a choice to the consumer, it is necessary to detect and label GM foods. This study was carried out to detect genetically modified tomato samples purchased from different food markets of Turkey. PCR method was used to detect genetically modified insect resistant tomatoes. The DNAs of collected samples were isolated according...
The effect of extrusion on the functional components and in vitro lycopene bioaccessibility of tomato pulp added corn extrudates
Tonyalı, Bade; Şensoy, İlkay; Karakaya, Sibel (2016-01-01)
The effect of processing on functional ingredients and their in vitro bioaccessibility should be investigated to develop better food products. Tomato pulp was added as a functional ingredient to extrudates. The effects of extrusion on the functional properties of the extrudates and the in vitro bioaccessibility of lycopene were investigated. Two different temperature sets were applied during extrusion: 80 degrees C, 90 degrees C, 100 degrees C and 130 degrees C and 80 degrees C, 100 degrees C, 130 degrees C...
Development of analysis methods for cry1ac and sam-k gene lines in tomato using pcr and real-time pcr
Uygun, Sahra; Gültekin, Güzin Candan; Department of Biotechnology (2010)
Genetically modified organisms are entering the human diet in all over the world. In order to have transparency in the foods that are being consumed, there is a need to trace the genetically modified organisms (GMOs) in the market and consequently this need brings the necessity of analytical methods that are capable of detecting, identifying and quantifying the transgenic events. These analytical methods also form the basis of the labeling regulations that are tried to be formed regarding GMOs. The main aim...
Citation Formats
G. C. Gültekin and R. Yilmaz, “Qualitative detection of GM maize (Bt 11) in food and feed sold commercially in Turkey by PCR based methods,” INDIAN JOURNAL OF BIOTECHNOLOGY, pp. 143–146, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/56016.