Detection of genetically modified insect resistant tomato via polymerase chain reaction

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2004

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Sönmezalp, C. Zeynep

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Tomato, which is one of the most important component of human diet, has been genetically modified to develop some properties like delayed ripening and insect resistance. In order to give a choice to the consumer, it is necessary to detect and label GM foods. This study was carried out to detect genetically modified tomato samples purchased from different food markets of Turkey. PCR method was used to detect genetically modified insect resistant tomatoes. The DNAs of collected samples were isolated according to CTAB DNA extraction protocol and the amplification capacity of isolated samples were checked with patatin specific control PCR. Screening tests of tomatoes were done by targeting 35S promoter, Nos terminator and NptII kanamycin resistance gene with four primer sets. It was aimed to detect Cry1A and Cry1Ac genes with three PCR systems, in order to identify insect resistant samples. From twenty-eight samples, twenty-two gave positive amplification signal in NptII specific PCR system and results were confirmed with sequence analysis. Additionally, we observed seventeen and ten DNA fragments with Cry1A-F/Cry1A-R and Cry1Ac-F/Cry1Ac-R primer sets respectively, it is necessary to confirm these results with DNA sequencing.

Subject Keywords

Genetics.

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http://etd.lib.metu.edu.tr/upload/3/12605493/index.pdf
https://hdl.handle.net/11511/14352

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

C. Z. Sönmezalp, “Detection of genetically modified insect resistant tomato via polymerase chain reaction,” M.S. - Master of Science, Middle East Technical University, 2004.