Evaluation of high hydrostatic pressure effects on bovine red blood cells and platelets

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2009-01-01

Author

Ceylan, Cagatay
Severcan, Mete
Bozoglu, Faruk
Severcan, Feride

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The objective of this study was to investigate the effects of high hydrostatic pressure (HHP) on the stability of red blood cells (RBCs) and platelets. Bovine blood cells (n=5) were treated with the pressure of 55, 110, 154 and 220MPa at 25C for 5min. Light microscopy, atomic force microscopy and flow cytometry studies revealed that RBCs were morphologically stable up until the 220MPa pressure treatments, at which surface modifications were observed. The platelets were found to be less stable than RBCs. HHP application did not cause any significant change in the signal intensity, band area and frequency values of the infrared bands with the exception that a significant variation was observed in the area of the cholesterol band. No statistically significant variations were observed in the secondary structure elements due to HHP treatment according to the artificial neural network study based on the FTIR data.

Subject Keywords

Condensed Matter Physics

URI

https://hdl.handle.net/11511/57498

Journal

HIGH PRESSURE RESEARCH

DOI

https://doi.org/10.1080/08957950902941014

Collections

Department of Biology, Article

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Citation Formats

C. Ceylan, M. Severcan, F. Bozoglu, and F. Severcan, “Evaluation of high hydrostatic pressure effects on bovine red blood cells and platelets,” HIGH PRESSURE RESEARCH, pp. 358–368, 2009, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57498.