Analysis of cellulase and polyphenol oxidase production by southern pine beetle associated fungi

Valiev, Abduvali
Ögel, Zümrüt Begüm
Klepzig, Kier D.
In this study, the production of extracellular enzymes by fungi associated with southern pine beetle was investigated for the first time. Cellulase and polyphenol oxidase production were analyzed for three beetle associated fungi. Only the mutualistic symbiont Entomocorticium sp. A was found to produce cellulases and polyphenol oxidase. In time course analyses of cellulase production in batch cultures, Entomocorticium sp. A showed maximum activity of 0.109 U/ml and 0.141 U/ml for total cellulase and endoglucanase activity respectively. Polyphenol oxidase production was simultaneous with fungal growth. Characterization of polyphenol oxidase by activity staining suggests that the enzyme is a tyrosinase/catechol oxidase. Enzyme assays in the presence of polyphenol oxidase inhibitors support the results of the activity staining.


Changes in Free Amino Acid and Sugar Levels of Dried Figs during Aflatoxin B(1) Production by Aspergillus flavus and Aspergillus parasiticus
Senyuva, Hamide Z.; Gilbert, John; Ozturkoglu, Sebnem; Özcan Kabasakal, Süreyya; Gurel, Neslihan (American Chemical Society (ACS), 2008-10-22)
An aqueous slurry of gamma-irradiated sterilized dried figs was inoculated with toxigenic strains of Aspergillus flavus and Aspergillus parasiticus. During incubation at 28 degrees C, pH, fructose, glucose, and free amino acids were determined by high-performance liquid chromatography and liquid chromatography (LC)/mass spectrometry, respectively, over 13 time points (1-20 days). At the same 13 time points using a LC/time-of-flight mass spectrometry screening method, aflatoxin B, and other secondary metabol...
ABDULLAH, MI; YOUNG, JC; GAMES, DE (American Chemical Society (ACS), 1994-03-01)
Material obtained by the CO2 supercritical fluid extraction (SFE) of the wild and cultivated mushrooms Agaricus silvicola and Agaricus bisporus, respectively, was analyzed by g chromatography (GC)-mass spectrometry and GC-Fourier transform infrared spectrometry. SFE gave results comparable with classical extraction techniques for free fatty acids and esters but with fewer manipulative steps and also resulted in the characterization of acids not previously reported from these sources. The organic acids ident...
Kinetic analysis of aspergillus oryzae cultivations on starch
Bayındırlı, Alev; UNGAN, S (Informa UK Limited, 1991-01-01)
Cultivation of Aspergillus oryzae on starch is described as a combination of two rate processes: Starch hydrolysis and the cellular activities of the fungi including growth, enzyme production and maintenance. Kinetic models are presented to describe growth, enzyme production, starch hydrolysis and uptake of the hydrolysis products. Numerical values of the model parameters indicated that the rate controlling step of A. oryzae growth on starch was not starch hydrolysis, but the substrate uptake process. Gluco...
Analysis of polyphenol oxidase production by southern pine beetle associated fungi
Valiev, Abduvali; Ögel, Zümrüt Begüm; Department of Food Engineering (2007)
In this study, two symbiotic fungi of Southern Pine Beetle (SPB), Entomocorticium peryii and Entomocorticium sp.A were evaluated in terms of polyphenol oxidase (PPO) production. The effect of different inhibitors, inducers and assay parameters such as temperature and pH on enzyme activity were investigated and maximum PPO activity was observed at 30°C, pH 8.0 and when tannic acid was used as an inducer. Copper-chelator salicyl hydroxamic acid (SHAM) and pcoumaric acid, both indicated as inhibitors of tyrosi...
Optimization of solid-liquid extraction of resveratrol and other phenolic compounds from milled grape canes (Vitis vinifera)
Karacabey, Erkan; Mazza, Giuseppe (American Chemical Society (ACS), 2008-08-13)
Optimization of the solid-liquid extraction conditions for trans-resveratrol, trans-E-viniferin, ferulic acid, and total phenolics from milled grape canes has been investigated. The temperature and ethanol concentration were found to be major process variables for all responses, whereas the solvent to solid ratio was found not to be significant for any of the responses studied. The yields of transresveratrol, trans-epsilon-viniferin, and total phenolics increased with increasing temperature. Maximum yields ...
Citation Formats
A. Valiev, Z. B. Ögel, and K. D. Klepzig, “Analysis of cellulase and polyphenol oxidase production by southern pine beetle associated fungi,” SYMBIOSIS, pp. 37–42, 2009, Accessed: 00, 2020. [Online]. Available: