Identification of differentially expressed transcripts from leaves of the boron tolerant plant Gypsophila perfoliata L.

Date

2008-08-01

Author

Unver, Turgay
Bozkurt, Osman
Akkaya, Mahinur

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

195
views

0
downloads

Very recently some of the species of Gypsophila genus collected from the boron rich soils in Turkey were shown to be remarkably tolerant to high levels of boron. A limited amount of boron is necessary for the normal development of plants; however, a high level of boron in soil is generally toxic. Nevertheless, the adaptability of plant species allows them to withstand the presence of extreme amounts of metal ion by various strategies. This study is conducted on highly boron tolerant Gypsophila perfoliata L. collected from a location in the boron mining area. The plant samples were transferred into plant nutritional medium in the presence high; similar to 500 (35 mg/kg), 1,000, and 30 mu M (considered normal) boron concentrations. We compared the transcriptome of the plant sample treated with the excess levels of boron to that of the samples grown under normal concentration using differential display PCR (DDRT-PCR) method. Thirty bands showing differential expression levels (presence or absence of bands or varying intensities) in either of similar to 500 or 30 mu M B concentrations at varying time points were excised, cloned, and sequenced. Among which, 18 of them were confirmed via quantitative reverse transcription real time PCR (qRT-PCR). We are reporting the first preliminary molecular level study of boron tolerance on this organism by attempting to identify putative genes related in the tolerance mechanism. The gene fragments are consistent with the literature data obtained from a proteomics study and a metabolomics study performed in barley under varying boron concentrations.

Subject Keywords

Agronomy and Crop Science, Plant Science, General Medicine

URI

https://hdl.handle.net/11511/57966

Journal

PLANT CELL REPORTS

DOI

https://doi.org/10.1007/s00299-008-0560-7

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Identification and characterization of hydrolytic enzymes from the midgut of Sunn Pest of wheat (Eurygaster integriceps) Ogur, E.; YÜCEL, MUSTAFA; Öktem, Hüseyin Avni (Informa UK Limited, 2009-01-01) To help in the development of Sunn Pest-resistant transgenic plants employing protease or alpha-amylase inhibitors, midgut hydrolytic enzymes of Sunn Pest (Eurygaster integriceps, Put.) (Heteroptera: Scutelleridae) were identified and characterized biochemically. We observed levels of very low proteolytic activity of trypsin (3 nmoles/min/mg), elastase (0.66 nmoles/min/mg) and leucine aminopeptidase-like (14.4 nmoles/min/mg) proteases, but no chymotrypsin and papain-like activity. Proteolytic activities wer...
Genetic relationships among perennial and annual Cicer species growing in Turkey as revealed by allozymes Sudupak, MA; Kence, Aykut (Springer Science and Business Media LLC, 2004-05-01) Allozyme polymorphisms were used to assess genetic variation and relationships among ten Cicer species ( annuals and perennials) growing in Turkey. Using seven enzyme systems, 12 putative scorable loci were detected and surveyed for polymorphism in an accession collection including wild and cultivated forms. Variation was generally low within accessions and species, but common between species. Cluster analysis based on the pairwise genetic distance coefficients (Nei 1978) among accessions and species using ...
Genetic relationships among perennial and annual Cicer species growing in Turkey assessed by AFLP fingerprinting Sudupak, MA; Akkaya, Mahinur; Kence, A (Springer Science and Business Media LLC, 2004-03-01) AFLP markers were used to assess genetic relationships among Cicer species with distribution in Turkey. Genetic distances were computed among 47 Cicer accessions representing four perennial and six annual species including chickpea, using 306 positions on AFLP gels. AFLP-based grouping of species revealed two clusters, one of which includes three perennial species, Cicer montbretii, Cicer isauricum and Cicer anatolicum, while the other cluster consists of two subclusters, one including one perennial, Cicer ...
A new taxon of the genus Salvia (Lamiaceae) from Turkey CELEP, FERHAT; Kahraman, Ahmet; Doğan, Musa (Botanical Garden Meise, 2011-01-01) Background and aims Turkey is one of the major centers of diversity for Salvia, with 97 species of which 54% are endemic. The aims of this study are to give detailed taxonomical, morphological and ecological accounts of S. cadmica and S. smyrnaea.
Characterization of taxonomically related some Turkish oak (quercus l.) species in an isolated stand : a morphometric analysis approach Aktaş, Caner; Kaya, Zeki; Department of Biology (2010) The genus Quercus L. is represented with more than 400 species in the world and 18 of these species are found naturally in Turkey. Although its taxonomical, phytogeographical and dendrological importance, the genus Quercus is still taxonomically one of the most problematical woody genus in Turkish flora. In this study, multivariate morphometric approach was used to analyze oak specimens collected from an isolated forest (Beynam Forest, Ankara) where Quercus pubescens Willd., Q. infectoria Olivier subsp. boi...

Citation Formats

T. Unver, O. Bozkurt, and M. Akkaya, “Identification of differentially expressed transcripts from leaves of the boron tolerant plant Gypsophila perfoliata L.,” PLANT CELL REPORTS, pp. 1411–1422, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57966.