Inhibitory effects of divalent metal ions on liver microsomal 7-ethoxyresorufin O-deethylase (EROD) activity of leaping mullet

Bozcaarmutlu, A
Arinc, E
The purpose of the present study was to elucidate in vitro effects of Hg2+, Zn2+, Ni2+ and Cd2+ on cytochrome P4501Al (CYP1A1) dependent EROD activities in leaping mullet liver microsomes. Fish captured from the most polluted part of Izmir Bay, had highly elevated EROD activities, and induced CYP1A1 protein levels as determined by Western blotting. Although all of the metal ions caused inhibition of the initial velocity of the reaction, Hg2+ and Cd2+ exhibited much higher inhibitory effect at lower concentrations and they were evidently more potent inhibitors than others. The inhibitor concentration giving 50% inhibition (IC50 values) of Zn2+, Ni2+, Cd2+ and Hg2+ of initial EROD activity were 107, 16, 1.3 and 0.15 micromolar, respectively. Glutathione (GSH) at 0.5 mM final concentration, completely reversed Ni2+ and Cd2+ inhibition of EROD activity indicating the protective action of GSH.


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Aim of this study was to determine the extent of pollution in the West Black Sea Coast of Turkey by measuring CYP1A associated EROD activity, phase II enzyme, glutathione S-transferase and antioxidant enzymes, catalase and glutathione reductase activities and immunochemical detection of CYP1A protein level in the liver of mullet. The fish samples were caught from six locations having a varying degree of pollution in the West Black Sea Region of Turkey in August 2005, 2006 and 2007. Mullets caught from Zongu...
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To date, no structural study has been carried out on the effects of vitamin A deficiency (VAD) on hippocampal macromolecules. Therefore, in the present study, the effect of dietary VAD on the structure, content and function of rat hippocampal molecules was investigated using Fourier transform IF spectroscopy. Male Wistar rats were randomly divided into three groups: an experimental group maintained on a vitamin A-deficient liquid diet (VAD, n 7); a control group maintained on a vitamin A-supplemented liquid...
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There are limited number of studies regarding the influence of diabetes on the regulation of cytochrome P450s and associated drug metabolizing enzyme activities especially in extrahepatic tissues such as kidney. However, there is almost no such study in lung. Alloxan-induced diabetes did not change CYP2B4 expression as measured with immunoblot analysis and associated enzyme, benzphetamine N-demethylase, activity in rabbit kidney and lung. Induction of cytochrome P4502E1 by diabetes was identified by immunoc...
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Objectives: In recent years, importance of enzyme activity measurements, in addition to genotyping, in epidemiological studies relating paraoxonase 1 (PON1) and vascular disease was emphasized. This is the first report evaluating paraoxonase and arylesterase activities as risk factors for ischemic stroke. In addition, PON1 192Gln(Q)/Arg(R) and 55Leu(L)/Met(M) polymorphisms were also analyzed.
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Information on temporal distribution of polychlorinated biphenyls (PCBs) in the coastal sediments of Mediterranean Sea, Mersin was compiled using data published between 1980 and 2009, and the present study. The first congener specific PCB results from the region yield concentration levels of Sigma(41) PCBs in sediments ranging from 0.61 to 1.04 ng g(-1). Sediment profiles show penta-, hexa- and hepta-chlorobiphenyls, specifically, #149 and 153 as the most abundant congeners in all samples. Comparison of tot...
Citation Formats
A. Bozcaarmutlu and E. Arinc, “Inhibitory effects of divalent metal ions on liver microsomal 7-ethoxyresorufin O-deethylase (EROD) activity of leaping mullet,” MARINE ENVIRONMENTAL RESEARCH, pp. 521–524, 2004, Accessed: 00, 2020. [Online]. Available: