Whole-cell fluorescent bacterial bioreporter for arsenic detection in water

Date

2019-10-01

Author

Elcin, E.
Oktem, H. A.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

174
views

0
downloads

Microbial whole-cell bioreporters have been developed for environmental monitoring of arsenic contamination. Despite the great interest in bacterial bioreporters for arsenite detection, relatively few studies reported their response to arsenate levels. In this study, green fluorescent protein-based whole-cell Escherichia coli bioreporter was constructed for the measurement of both bioavailable arsenite and arsenate in water. The developed bacterial bioreporter has much higher sensitivity toward arsenate in inorganic phosphate-limiting medium compared to minimal medium. Both arsenite and arsenate were detected at 10 mu g/l within 2-h induction time period. Furthermore, the bioreporter was able to detect and estimate arsenic levels in groundwater sample. The results demonstrate that the constructed bacterial arsenic bioreporter is applicable in determining the concentrations of the most abundant two ionic forms of arsenic as opposed to analytical methods which gives total content of arsenic. Thus, a comparison of bioavailability of arsenic and its form with its total content would give an insight about the risk of toxicity and also planning of removal processes.

Subject Keywords

Environmental Engineering, General Agricultural and Biological Sciences, Environmental Chemistry

URI

https://hdl.handle.net/11511/65375

Journal

INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY

DOI

https://doi.org/10.1007/s13762-018-2077-0

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Development of a fuzzy rule based remedial priority ranking system for contaminated sites Polat, Şener; Ünlü, Kahraman; Department of Environmental Engineering (2009) Evaluation of contaminated sites based on human health and environmental hazards is an essential task for the proper management of the contaminated sites. A large number of contaminated sites have been waiting for remediation all over the World. However, contaminated site remediation is generally a difficult, time consuming and very expensive process. Ranking systems for contaminated sites are useful tools to determine the remedial priority and to manage the available remediation budget in the most efficien...
Experimental Study on Maize Cob Trickling Filter-Based Wastewater Treatment System: Design, Development, and Performance Evaluation Ali, Imran; Khan, Zahid M.; Sultan, Muhammad; Mahmood, Muhammad H.; Farid, Hafiz U.; Ali, Mohsin; Nasir, Abdul (HARD Publishing Company, 2016-01-01) In developing countries, good-quality water is contaminated due to the disposal of untreated municipal and industrial wastewater (WW) into natural water reservoirs. Most of the wastewater is not treated properly according to international standards, and usually is disposed of and/or utilized for irrigation without appropriate treatment. The main hurdles in providing wastewater treatment (WWT) in developing countries include high costs, and the poor design, installation, and operation of conventional WWT sys...
Rapid and Concomitant Analysis. of Pharmaceuticals in Treated Wastewater by Coated Blade Spray Mass Spectrometry Poole, Justen J.; Gomez-Rios, German A.; Boyacı, Ezel; Reyes-Garces, Nathaly; Pawliszyn, Janusz (American Chemical Society (ACS), 2017-11-07) The widespread use of pharmaceuticals in both human and animal populations, and the resultant contamination of surface waters from the outflow of water treatment facilities is an issue of growing concern. This has raised the need for analytical methods that can both perform rapid sample analysis and overcome the limitations of conventional analysis procedures, such as multistep workflows and tedious procedures. Coated blade spray (CBS) is a solid-phase microextraction based technique that enables the direct...
Assessment of PCB contamination, the potential for in situ microbial dechlorination and natural attenuation in an urban watershed at the East Coast of the United States Kaya, Devrim; Sowers, Kevin R.; Demirtepe, Hale; Stiell, Brian; Baker, Joel E.; İmamoğlu, İpek; Kjellerup, Birthe (Elsevier BV, 2019-09-15) Sediment contamination is a major environmental issue in many urban watersheds and coastal areas due to the potential toxic effects of contaminants on biota and human health. Characterizing and delineating areas of sediment contamination and toxicity are important goals of coastal resource management in terms of ecological and economical perspectives. Core and surficial sediment samples were collected from an industrialized urban watershed at the East Coast of the United Stated and analyzed to evaluate the ...
Evaluation of Turkey's status in terms of compliance with the requirements of the Stockholm convention Şıltu, Esra; İmamoğlu, İpek; Department of Environmental Engineering (2014) Persistent Organic Pollutants (POPs) are stable, toxic compounds released into the environment due to anthropogenic activities. POPs can accumulate in fatty tissues of living organisms and become concentrated up the food chain, posing a serious threat to environment and human health. Once they are released, POPs participate in long-range transport and can have a widespread effect. Stockholm Convention on POPs, which was adopted in 2001 and entered into force in 2004, is a global treaty to take measures agai...

Citation Formats

E. Elcin and H. A. Oktem, “Whole-cell fluorescent bacterial bioreporter for arsenic detection in water,” INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY, pp. 5489–5500, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/65375.