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The effect of crosslinking on thermal inactivation of cellulases
Date
1998-04-22
Author
Bilen, J
Bakir, U
Metadata
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Cellulase complex was crosslinked by using three different crosslinkers, dimethyl suberimidate (DMS), dimethyl adipimidate (DMA) and dimethyl-3-3'-dithiobispropionimidate (DTBP) and their effects on thermal inactivation were investigated. These reagents are all homobifunctional bisimidoesters having different crosslinking distances and crosslink only amino groups to each other. No significant activity loss was observed due to crosslinking with none of the crosslinkers used. In general, DMA and DTBP crosslinking decreased the thermal stability slightly, however with DMS crosslinking, slight increases in thermal stabilities were observed.
Subject Keywords
Protein
URI
https://hdl.handle.net/11511/65758
Collections
Department of Biology, Conference / Seminar
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J. Bilen and U. Bakir, “The effect of crosslinking on thermal inactivation of cellulases,” 1998, vol. 15, p. 151, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/65758.
