The effects of microbial lignocellulose pretreatments on xylooligosaccharide production

Uckun, E.
Ak, O.
Bakir, U.
Lignocellulosic agricultural waste materials are produced in huge amounts in the world and regarded as abundant, inexpensive readily available natural sources for various industries. They are mainly composed of three types of macromolecules, cellulose, hemicellulose and lignin. Cellulose and hemicellulose are converted to a variety of valuable compounds such as enzymes, hormones, antibiotics, ethanol, sugar alcohols, organic acids and xylooligosaccharides (XOs) using bioprocessing methods easily after the removal of lignin from the structure. XOs are a new group of oligosaccharides that are gaining importance as functional food ingredients in pharmaceuticals, feed formulations and agriculture. They act as prebiotics promoting the growth of beneficial Bifidobacteria in the colon, reducing the risk of colon cancer. The major purpose of this study is to determine the effect of laccase enzyme and a white rot fungi, Phanerochaete chrysosporium, pretreatment to improve XO production from cotton stalks, which are found in huge amounts in Turkey and has no economical value. Because xylan extraction using chemical methods is not only expensive but also not environmental friendly, XOs are produced from biologically pretreated cotton stalks using controlled enzymatic hydrolysis. During enzymatic hydrolysis of lignocellulosic materials, the effect of the medium pH, temperature and the concentration of pretreated or unpretreated cotton stalks and enzymes on the production of reducing sugar were investigated using DNSA method. XOs and other compounds present in the hydrolyzates were characterized by TLC and HPLC.


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Citation Formats
E. Uckun, O. Ak, and U. Bakir, “The effects of microbial lignocellulose pretreatments on xylooligosaccharide production,” NEW BIOTECHNOLOGY, pp. 0–0, 2009, Accessed: 00, 2020. [Online]. Available: