Inhibition mechanisms of lead, cadmium and nickel for the purified bovine liver NADPH-cytochrome P450 reductase

Date

2011-08-28

Author

BOZCAARMUTLU, AZRA
Celik, H.
Arinc, E.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

178
views

0
downloads

Very little information is available on the effects of metals in veterinary animals, despite the fact that they are exposed to these chemicals via contaminated plants and water sources. NADPH-Cytochrome P450 reductase is an essential component of the microsomal cytochrome P450 monooxygenase system. In this study, our aim was to elucidate in vitro effect of Pb2+, Cd2+ and Ni2+ on the purified bovine NADPH-cytochrome P450 reductase. NADPH-cytochrome P450 reductase was purified from bovine liver microsomes. The effects of metals were studied by adding various concentrations of them on the reaction mixture. All of the metal ions caused inhibition of the enzyme activity. The IC50 values of Pb2+, Cd2+ and Ni2+ were estimated to be 0.027 mM, 0.059 mM and 2.9 mM, respectively. Of the metal ions tested, Pb2+ exhibited much higher inhibitory effect at lower concentrations. Kinetic analysis was done to determine type of inhibition of purified bovine cytochrome P450 reductase. All of the metal ions displayed noncompetitive type of inhibition mechanism for the purified NADPH-cytochrome P450 reductase as analyzed by Lineweaver-Burk Plot and Dixon plot. Ki values of Pb2+, Cd2+ and Ni2+ were calculated from Dixon plots as 0.03 mM, 0.04 mM and 2.0 mM, respectively. The results of this study clearly indicate that mechanism of inhibition of lead, cadmium and nickel for cytochrome P450 reductase in bovine is noncompetitive type.

Subject Keywords

Toxicology, General Medicine

URI

https://hdl.handle.net/11511/66632

DOI

https://doi.org/10.1016/j.toxlet.2011.05.664

Collections

Department of Biology, Conference / Seminar

Suggestions

OpenMETU
Core

Characterization of aldrin epoxidation in flathead mullet (Mugil cephalus) liver microsomes: Involvement of CYP3A BOZCAARMUTLU, AZRA; Turna, S.; Erden, C. Sapmaz; Yenisoy-Karakas, S.; Arinc, E. (2011-08-28) Toxic organochlorine pesticides, aldrin and its metabolite dieldrin, have been reported to contaminate the aquatic environment highly. In the present study, epoxidation reaction of aldrin to dieldrin was studied in mullet liver microsomes and contribution of cytochrome P450 isozyme(s) was determined by using specific cytochrome P450 inhibitors and substrate. Flathead mullet samples (Mugil cephalus) were caught from the West Black Sea Region of Turkey. Fish liver microsomes were prepared by differential cent...
Exposure to a microplastic mixture is altering the life traits and is causing deformities in the non-biting midge Chironomus riparius Meigen (1804) Stankovic, Jelena; Milosevic, Djuradj; Savic-Zdrakovic, Dimitrija; Yalçın, Gülce; Yildiz, Dilvin; Beklioğlu, Meryem; Jovanovic, Boris (Elsevier BV, 2020-07-01) The effect of microplastics (MP) exposure on the chironomid species Chironomus riparius Meigen, 1804 was investigated using the OECD sediment and water toxicity test. Chironomid larvae were exposed to an environmentally relevant low microplastics concentration (LC), a high microplastics concentration (HC) and a control (C). The LC was 0.007 g m(-2) on the water surface + 2 g m(-3) in the water column + 8 g m(-2) in the sediment, and the HC was 10 X higher than this for each exposure. The size of the majorit...
Investigation of the role of glutathione S-transferase isozymes in pyrethroid resistance of Helicoverpa armigera in Turkey Konus, Metin; Ugurlu, Sakine; İşcan, Mesude (2009-09-13) Helicoverpa armigera, cotton bollworm, is one of the insects causing severe yield loss of economically important crops including cotton, legumes and vegetables. It has developed resistance against the pyrethroid insecticides that have been introduced into market. Resistance against pyrethroids have been reported in H. armigera populations at various parts of the world, however, except 1-chloro-2,4-dinitrobenzene substrate (CDNB), almost no any other substrates used for analysis of the role of glutathione S-...
Use of cadA-Specific Primers and DNA Probes as Tools to Select Cadmium Biosorbents with Potential in Remediation Strategies İçgen, Bülent (Springer Science and Business Media LLC, 2016-05-01) Biosorption, using cadmium-resistant bacterial isolates, is often regarded as a relatively inexpensive and efficient way of cleaning up wastes, sediments, or soils polluted with cadmium. Therefore, many efforts have been devoted to the isolation of cadmium-resistant isolates for the efficient management of cadmium remediation processes. However, isolation, identification and in situ screening of efficient cadmium-resistant isolates are primary challenges. To overcome these challanges, in this study, cadA, c...
Biofilms attached to Myriophyllum spicatum play a dominant role in nitrogen removal in constructed wetland mesocosms with submersed macrophytes: Evidence from N-15 tracking, nitrogen budgets and metagenomics analyses Mu, Xiaoying; Lv, Xiaoyang; Liu, Wei; Qiu, Changhao; Ma, Yu; Zhang, Songhe; Jeppesen, Erik (Elsevier BV, 2020-11-01) The mechanisms behind nitrogen removal by the submersed macrophyte-biofilm complex in wetlands remain to be fully elucidated. This study investigated the role of Myriophyllum spicatum and the biofilm on their leaves in nitrogen removal in mesocosm experiments. N-15 tracking showed that 61.9% and 30% of the N-15, respectively, was removed from the system and assimilated by the macrophyte-biofilm complex after loading with 5.4 mg L-1 N-15 labelled NH4+ for 17 days. Nitrogen budget results showed that about 0....

Citation Formats

A. BOZCAARMUTLU, H. Celik, and E. Arinc, “Inhibition mechanisms of lead, cadmium and nickel for the purified bovine liver NADPH-cytochrome P450 reductase,” 2011, vol. 205, p. 0, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/66632.