Investigation of the role of glutathione S-transferase isozymes in pyrethroid resistance of Helicoverpa armigera in Turkey

Konus, Metin
Ugurlu, Sakine
İşcan, Mesude
Helicoverpa armigera, cotton bollworm, is one of the insects causing severe yield loss of economically important crops including cotton, legumes and vegetables. It has developed resistance against the pyrethroid insecticides that have been introduced into market. Resistance against pyrethroids have been reported in H. armigera populations at various parts of the world, however, except 1-chloro-2,4-dinitrobenzene substrate (CDNB), almost no any other substrates used for analysis of the role of glutathione S-transferases (GST) in pyrethroid resistance of H. armigera. In this study, in addition to CDNB, GST activities were determined using 4-nitrobenzylchloride (PNBC), 3,4-dichloronitrobenzene (DCNB) and 1,2-epoxy-3-(p-nitrophenoxy)propane (EPNP) as substrates. Midguts of H. armigera obtained from Çanakkale (n = 30) and Mardin (n = 30) field populations and a susceptible (n = 30) population obtained from Germany. GST-PNBC activities in Çanakkale and Mardin field populations showed statistically significant (p < 0.05), 2.2- and 4.8-fold increase, respectively, compared to susceptible population. Similarly, GST-DCNB activities showed statistically significant (p < 0.05), 2.0- and 4.8-fold increase in Çanakkale and Mardin field populations, respectively. However, as GST activities of both populations were determined using common substrate CDNB, only Mardin field population showed statistically significant (p < 0.05), 1.5-fold increase compared to susceptible population, no change in the GST-CDNB activity was observed in Çanakkale field population. Finally, GST activities against EPNP in Çanakkale and Mardin field populations were not detected. Thus, it may be concluded that GST isozymes using DCNB and PNBC as substrates play a role in pyrethroid resistance of Çanakkale and Mardin field populations.


Characterization of aldrin epoxidation in flathead mullet (Mugil cephalus) liver microsomes: Involvement of CYP3A
BOZCAARMUTLU, AZRA; Turna, S.; Erden, C. Sapmaz; Yenisoy-Karakas, S.; Arinc, E. (2011-08-28)
Toxic organochlorine pesticides, aldrin and its metabolite dieldrin, have been reported to contaminate the aquatic environment highly. In the present study, epoxidation reaction of aldrin to dieldrin was studied in mullet liver microsomes and contribution of cytochrome P450 isozyme(s) was determined by using specific cytochrome P450 inhibitors and substrate. Flathead mullet samples (Mugil cephalus) were caught from the West Black Sea Region of Turkey. Fish liver microsomes were prepared by differential cent...
Use of cadA-Specific Primers and DNA Probes as Tools to Select Cadmium Biosorbents with Potential in Remediation Strategies
İçgen, Bülent (Springer Science and Business Media LLC, 2016-05-01)
Biosorption, using cadmium-resistant bacterial isolates, is often regarded as a relatively inexpensive and efficient way of cleaning up wastes, sediments, or soils polluted with cadmium. Therefore, many efforts have been devoted to the isolation of cadmium-resistant isolates for the efficient management of cadmium remediation processes. However, isolation, identification and in situ screening of efficient cadmium-resistant isolates are primary challenges. To overcome these challanges, in this study, cadA, c...
Roles of detoxification enzymes in pyrethroid resistance of Helicoverpa armigera from Turkey
Konus, Metin; UgurIci, Sakine; İşcan, Mesude (2008-10-05)
Abstracts / Toxicology Letters 180S (2008) S32–S246S169Helicoverpa armigera, cotton bollworm, is a polyphagous insect. Itis one of the most important preharvest pests for economicallyimportant crops including cotton, legumes, and vegetables.In this study, midguts ofH. armigeraobtained from Adana,Antalya, C ̧ anakkale and Mardin field populations were used. GSTactivities were determined using 1-chloro-2,4-dinitrobenzene asa substrate. Adana and Antalya field populations showed statisti-cally ...
Production and characterization of xylanases of a Bacillus strain isolated from soil
Avcioglu, B; Eyupoglu, B; Bakir, U (Springer Science and Business Media LLC, 2005-02-01)
Xylanase production was performed by growing a Bacillus isolate on agricultural by-products, wheat straw, wheat bran, corn cobs and cotton bagasse. A maximum xylanase activity of 180 U/ml was obtained together with a cellulase activity of 0.03 U/ml on 4 (w/v) corn cobs. Electrophoretic analysis showed the presence of three endo-beta-1, 4-xylanases having molecular weights of about 22, 23 and 40 kDa. Xylanolytic activity was stable up to 50degreesC in the pH range of 4.5-10 and the highest activity was obser...
Development of in vitro micropropagation techniques for saffron (Crocus sativus L.)
Yıldırım, Evrim; Kaya, Zeki; Department of Biology (2007)
In vitro micropropagation of saffron (Crocus sativus L.) by using direct and indirect organogenesis was the aim of this study. Also, the effect of plant growth regulators on growth parameters, such as corm production, sprouting time and germination ratio were investigated in ex vitro conditions. For in vitro regeneration of saffron, the effects of 2,4-D (2,4-dichlorophenoxyacetic acid) and BAP (6-benzylaminopurine) were tested initially. It was observed that 0,25 mg/L 2,4-D and 1 mg/L BAP combination was su...
Citation Formats
M. Konus, S. Ugurlu, and M. İşcan, “Investigation of the role of glutathione S-transferase isozymes in pyrethroid resistance of Helicoverpa armigera in Turkey,” 2009, vol. 189, Accessed: 00, 2020. [Online]. Available: