Detection of porcine DNA in gelatine and gelatine-containing processed food products-Halal/Kosher authentication

Date

2012-03-01

Author

Demirhan, Yasemin
Ulca, Pelin
Senyuva, Hamide Z.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

22
views

0
downloads

A commercially available real-time PCR, based on a multi-copy target cytochrome b (cyt b) using porcine specific primers, has been validated for the Halal/Kosher authentication of gelatine. Extraction and purification of DNA from gelatine were successfully achieved using the Surefood (R) PREP Animal system, and real-time PCR was carried out using SureFood (R) Animal ID Pork Sens kit. The minimum level of adulteration that could be detected was 1.0% w/w for marshmallows and gum drops. A small survey was undertaken of processed food products such as gum drops, marshmallows and Turkish delight, believed to contain gelatine. Of fourteen food products from Germany, two samples were found to contain porcine gelatine, whereas of twenty-nine samples from Turkey twenty-eight were negative. However, one product from Turkey contained porcine DNA and thus was not Halal, and neither was the use of porcine gelatine indicated on the product label.

Subject Keywords

Gelatine, Halal authentication, Kosher authentication, Real-time PCR

URI

https://hdl.handle.net/11511/67235

Journal

MEAT SCIENCE

DOI

https://doi.org/10.1016/j.meatsci.2011.10.014

Collections

Unclassified, Article

Suggestions

OpenMETU
Core

Mapping electrically active dopant profiles by field-emission scanning electron microscopy Turan, Raşit; Houghton, DC (1996-09-09) Secondary electron (SE) image contrast from p-type silicon has been studied using field-emission scanning electron microscopy (FE-SEM). Cross-sectional FE-SEM images of boron-doped silicon heterostructures have been compared with atomic concentration and free carrier profiles measured by secondary-ion mass spectroscopy and electrochemical capacitance-voltage profiling, respectively. FE-SEM image contrast due to dopants has been shown to be electronic in origin. Since electrically active dopant species contr...
Analysis of the genetic determinant for production of the pediocin P of Pediococcus pentosaecus Pep 1 Osmanagaoglu, O; Beyatli, Y; Gündüz, Ufuk; Sacilik, SC (2000-01-01) Pediococcus pentosaceus Pep1 is a vacuum-packaged Turkish sausage isolate which produces a potentially novel bacteriocin of the pediocin (anti-Listeria) family of peptides designated as pediocin P. Curing experiments and plasmid profile analysis indicated that both bacteriocin immunity and production determinants were linked and encoded by 9.0 MDa plasmid, pHD1.0. Attempts to transform purified plasmid pHD1.0 into recipient Escherichia coli JM109 cells by electroporation were successful but none of the E. c...
Preparation of silica coated cobalt ferrite magnetic nanoparticles for the purification of histidine-tagged proteins Aygar, Gulfem; Kaya, Murat; Özkan, Necati; Kocabıyık, Semra; Volkan, Mürvet (2015-12-01) Surface modified cobalt ferrite (CoFe2O4) nanoparticles containing Ni-NTA affinity group were synthesized and used for the separation of histidine tag proteins from the complex matrices through the use of imidazole side chains of histidine molecules. Firstly, CoFe2O4 nanoparticles with a narrow size distribution were prepared in an aqueous solution using the controlled co-precipitation method. In order to obtain small CoFe2O4 agglomerates, oleic acid and sodium chloride were used as dispersants. The CoFe2O4...
Determination of fumonisins B-1 and b2 in corn by liquid chromatography/mass spectrometry with immunoaffinity column cleanup: Single-laboratory method validation Senyuva, Hamide Z.; Özcan Kabasakal, Süreyya; Cimen, Dilek; Gilbert, John (2008-05-01) A single-laboratory method validation was conducted to establish the effectiveness of an immunoaffinity column cleanup procedure followed by liquid chromatography/mass spectrometry (LC/MS) for the determination of fumonisins B-1 and B-2 (FB1 + FB2) in corn. The test portion is extracted with acetonitrile-methanol-water (25 + 25 + 50). The extract is filtered, diluted with phosphate-buffered saline solution, and applied to an immunoaffinity column. FB1 + FB2 are removed with methanol and directly determined ...
Identification of ligand binding regions of the Saccharomyces cerevisiae alpha-factor pheromone receptor by photoaffinity cross-linking Son, Çağdaş Devrim; Naider, F; Becker, JM (American Chemical Society (ACS), 2004-10-19) Analogues of alpha-factor, Saccharomyces cerevisiae tridecapeptide mating pheromone (H-Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr-OH), containing p-benzoylphenylalanine (Bpa), a photoactivatable group, and biotin as a tag, were synthesized using solid-phase methodologies on a p-benzyloxybenzyl alcohol polystyrene resin. Bpa was inserted at positions 1, 3, 5, 8, and 13 of alpha-factor to generate a set of cross-linkable analogues spanning the pheromone. The biological activity (growth arrest assay) ...

Citation Formats

Y. Demirhan, P. Ulca, and H. Z. Senyuva, “Detection of porcine DNA in gelatine and gelatine-containing processed food products-Halal/Kosher authentication,” MEAT SCIENCE, pp. 686–689, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/67235.