Preparation of silica coated cobalt ferrite magnetic nanoparticles for the purification of histidine-tagged proteins

Date

2015-12-01

Author

Aygar, Gulfem
Kaya, Murat
Özkan, Necati
Kocabıyık, Semra
Volkan, Mürvet

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Surface modified cobalt ferrite (CoFe2O4) nanoparticles containing Ni-NTA affinity group were synthesized and used for the separation of histidine tag proteins from the complex matrices through the use of imidazole side chains of histidine molecules. Firstly, CoFe2O4 nanoparticles with a narrow size distribution were prepared in an aqueous solution using the controlled co-precipitation method. In order to obtain small CoFe2O4 agglomerates, oleic acid and sodium chloride were used as dispersants. The CoFe2O4 particles were coated with silica and subsequently the surface of these silica coated particles (SiO2-CoFe2O4) was modified by amine (NH2) groups in order to add further functional groups on the silica shell. Then, carboxyl (-COOH) functional groups were added to the SiO2-CoFe2O4 magnetic nanoparticles through the NH2 groups. After that N alpha,N alpha-Bis(carboxymethyl)-L-lysine hydrate (NTA) was attached to carboxyl ends of the structure. Finally, the surface modified nanoparticles were labeled with nickel (Ni) (II) ions. Furthermore, the modified SiO2-CoFe2O4 magnetic nanoparticles were utilized as a new system that allows purification of the N-terminal His-tagged recombinant small heat shock protein, Tpv-sHSP 14.3.

Subject Keywords

Magnetic nanoparticles, Magnetic separation, Surface modification, Small heat-shock protein, His-tagged protein

URI

https://hdl.handle.net/11511/31354

Journal

JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS

DOI

https://doi.org/10.1016/j.jpcs.2015.08.005

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

G. Aygar, M. Kaya, N. Özkan, S. Kocabıyık, and M. Volkan, “Preparation of silica coated cobalt ferrite magnetic nanoparticles for the purification of histidine-tagged proteins,” JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS, pp. 64–71, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31354.