Preparation of silica coated cobalt ferrite magnetic nanoparticles for the purification of histidine-tagged proteins

Aygar, Gulfem
Kaya, Murat
Özkan, Necati
Kocabıyık, Semra
Volkan, Mürvet
Surface modified cobalt ferrite (CoFe2O4) nanoparticles containing Ni-NTA affinity group were synthesized and used for the separation of histidine tag proteins from the complex matrices through the use of imidazole side chains of histidine molecules. Firstly, CoFe2O4 nanoparticles with a narrow size distribution were prepared in an aqueous solution using the controlled co-precipitation method. In order to obtain small CoFe2O4 agglomerates, oleic acid and sodium chloride were used as dispersants. The CoFe2O4 particles were coated with silica and subsequently the surface of these silica coated particles (SiO2-CoFe2O4) was modified by amine (NH2) groups in order to add further functional groups on the silica shell. Then, carboxyl (-COOH) functional groups were added to the SiO2-CoFe2O4 magnetic nanoparticles through the NH2 groups. After that N alpha,N alpha-Bis(carboxymethyl)-L-lysine hydrate (NTA) was attached to carboxyl ends of the structure. Finally, the surface modified nanoparticles were labeled with nickel (Ni) (II) ions. Furthermore, the modified SiO2-CoFe2O4 magnetic nanoparticles were utilized as a new system that allows purification of the N-terminal His-tagged recombinant small heat shock protein, Tpv-sHSP 14.3.


Synthesis of Optically Complex, Porous, and Anisometric Polymeric Microparticles by Templating from Liquid Crystalline Droplets
Wang, Xiaoguang; Büküşoğlu, Emre; Miller, Daniel S; Pantoja, Marco A. Bedolla; Xiang, Jie; Lavrentovich, Oleg D; Abbott, Nicholas L (Wiley, 2016-10-25)
It is demonstrated that aqueous dispersions of micrometer-sized liquid crystal (LC) droplets provide the basis of a general and facile methodology for the templated synthesis of spherical and nonspherical polymeric micro-particles with complex internal structure and porosity. Specifically, nematic droplets of reactive (RM257)/nonreactive mesogens with distinct internal configurations are prepared using a range of approaches, the reactive mesogens are photopolymerized, and then the nonreactive mesogens are e...
Synthesis of New Thienylene Pyrrole Monomers and Their Electropolymerizations
Eken, Serap; Carbas, Buket Bezgin; Akdağ, Akın; Önal, Ahmet Muhtar (The Electrochemical Society, 2014-01-01)
In this study, four new 2,5-dithienylpyrroles (SNS) 1-(3-fluoro-phenyl)-2,5-di(thiophen-2-yl)-1H-pyrrole (m-FPTP), 1-(3-chlorophenyl)-2,5-di(thiophen-2-yl)-1H-pyrrole (m-ClPTP), 1-(3-bromo-phenyl)-2,5-di(thiophen-2-yl)-1H-pyrrole (m-BrPTP) and 1-(4-bromo-phenyl)-2,5-di(thiophen-2-yl)-1H-pyrrole (p-BrPTP) containing meta and para substituted benzene ring at the nitrogen atom were synthesized via Paal-Knorr condensation of 1,4-di(2-thienyl)-1,4-butanedione with corresponding halo-substituted anilines. The mon...
Application of the Ugi reaction with multiple amino acid-derived components: synthesis and conformational evaluation of piperazine-based minimalist peptidomimetics.
Stucchi, Mattia; Cairati, Silvia; Atalay, Rengül; Christodoulou, Michael S.; Grazioso, Giovanni; Pescitelli, Gennaro; Silvani, Alessandra; Kahraman, Deniz Cansen; Lesma, Giordano (2015-05-07)
The concurrent employment of alpha-amino acid-derived chiral components such as aldehydes and alpha-iso-cyanoacetates, in a sequential Ugi reaction/cyclization two-step strategy, opens the door to the synthesis of three structurally distinct piperazine-based scaffolds, characterized by the presence of L-Ala and/or L-Phe-derived side chains and bearing appropriate functionalities to be easily applied in peptide chemistry. By means of computational studies, these scaffolds have been demonstrated to act as min...
Effect of impact modifier on the properties of poly (ethylene terephthalate)-organoclay nanocomposites
Yılmazer, Ülkü; Alyamac, Elif (2006-11-30)
The effects of component concentrations and addition order of the components, on the final properties of ternary nanocomposites composed of amorphous poly (ethylene terephthalate) matrix, organically modified clay, and an ethylene / methyl acrylate / glycidyl methacrylate (E-MAGMA) terpolymer were studied. All formulations were prepared by melt compounding of the components with a two-step mixing procedure in a corotating twin-screw extruder. Considering the X-Ray Diffraction, SEM, impact and strain at brea...
Preparation of Chitosan-Coated Magnetite Nanoparticles and Application for Immobilization of Laccase
Kalkan, Nuzhet Ayca; AKSOY, SERPİL; Aksoy, Eda Ayse; Hasırcı, Nesrin (2012-01-15)
In this study, immobilization of laccase (L) enzyme on magnetite (Fe(3)O(4)) nanoparticles was achieved, so that the immobilized enzyme could be used repeatedly. For this purpose, Fe(3)O(4) nanoparticles were coated and functionalized with chitosan (CS) and laccase from Trametes versicolor was immobilized onto chitosan-coated magnetic nanoparticles (Fe(3)O(4)-CS) by adsorption or covalent binding after activating the hydroxyl groups of chitosan with carbodiimide (EDAC) or cyanuric chloride (CC). For chitosa...
Citation Formats
G. Aygar, M. Kaya, N. Özkan, S. Kocabıyık, and M. Volkan, “Preparation of silica coated cobalt ferrite magnetic nanoparticles for the purification of histidine-tagged proteins,” JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS, pp. 64–71, 2015, Accessed: 00, 2020. [Online]. Available: