Determination of fumonisins B-1 and b2 in corn by liquid chromatography/mass spectrometry with immunoaffinity column cleanup: Single-laboratory method validation

Date

2008-05-01

Author

Senyuva, Hamide Z.
Özcan Kabasakal, Süreyya
Cimen, Dilek
Gilbert, John

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A single-laboratory method validation was conducted to establish the effectiveness of an immunoaffinity column cleanup procedure followed by liquid chromatography/mass spectrometry (LC/MS) for the determination of fumonisins B-1 and B-2 (FB1 + FB2) in corn. The test portion is extracted with acetonitrile-methanol-water (25 + 25 + 50). The extract is filtered, diluted with phosphate-buffered saline solution, and applied to an immunoaffinity column. FB1 + FB2 are removed with methanol and directly determined by reversed-phase LC with MS detection using selected-ion monitoring of 2 characteristic ions in each case. Test portions of blank corn samples were spiked with a mixture of FB1 + FB2 to give total levels of 200 and 500 ng/g, respectively. Recoveries of both FB1 and FB2 from spiked samples averaged 90.4-101%. Based on results for spiked raw corn (triplicates at 2 levels), the relative standard deviation for repeatability ranged from 2.8 to 7.1%. The accuracy of the method was demonstrated by analysis of Food Analysis Performance Assessment Scheme (FAPAS (R)) test material. The method was also applied to a small survey of processed corn products such as corn chips, cornflakes, and popcorn.

Subject Keywords

Tandem mass-spectrometry, Fusarium mycotoxins, Foods, Maize, Products, Consumption, Extraction, Cereals

URI

https://hdl.handle.net/11511/54648

Journal

JOURNAL OF AOAC INTERNATIONAL

Collections

Department of Chemistry, Article

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Citation Formats

H. Z. Senyuva, S. Özcan Kabasakal, D. Cimen, and J. Gilbert, “Determination of fumonisins B-1 and b2 in corn by liquid chromatography/mass spectrometry with immunoaffinity column cleanup: Single-laboratory method validation,” JOURNAL OF AOAC INTERNATIONAL, pp. 598–606, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/54648.