The effect of seed size on tissue culture response of callus from endosperm-supported mature embryos of barley (Hordeum vulgare L.)

2007-09-01
Oezgen, M.
Yildiz, M.
Koyuncu, N.
Oende, S.
Although immature embryos are the most suitable explants to regenerate cereals, their limited availability throughout the year hampers frequent experimentation. In order to circumvent this problem, endosperm-supported mature embryos, which are available throughout the year, are proposed for utilization in cereal tissue culture studies. In the present study, the effect of seed size on in vitro seed germination, seedling growth, callus induction and plant regeneration, as well as the relationships between these parameters, were investigated in three barley (Hordewn vidgare L.) genotypes. Seeds were designated as large or small for each genotype and seed germinations were achieved in Petri dishes between two sheets of pre-wetted filter paper. Germination percentages of seeds, seedling growth rates, shoot and root lengths, and seedling fresh and dry weights were examined. Mature embryos from imbibed and dehulled seeds were aseptically moved slightly with a scalpel. For callus induction, 8 mg 1(-1) 2,4-D dissolved in water, and for plant regeneration, hormone-free MS medium, was utilized. Highest values with respect to seed germination percentage, seedling growth, callus induction and plant regeneration were obtained using large seeds. Rooting of regenerated shoots and plantlet recovery were also successfully achieved. Based on the significant positive correlations observed between all parameters, we concluded that cultures derived from endosperm -supported mature embryos within large seeds should be employed for achieving superior tissue culture response.
CEREAL RESEARCH COMMUNICATIONS

Suggestions

Detection of genetically modified insect resistant tomato via polymerase chain reaction
Sönmezalp, C. Zeynep; Gültekin, Güzin Candan; Department of Biotechnology (2004)
Tomato, which is one of the most important component of human diet, has been genetically modified to develop some properties like delayed ripening and insect resistance. In order to give a choice to the consumer, it is necessary to detect and label GM foods. This study was carried out to detect genetically modified tomato samples purchased from different food markets of Turkey. PCR method was used to detect genetically modified insect resistant tomatoes. The DNAs of collected samples were isolated according...
Analysis of self-processing mechanism of galactose oxidase by site-directed mutagenesis and heterologous expression in Escherichia Coli
Gençer, Burçak; Ögel, Zümrüt Begüm; Department of Biotechnology (2005)
In this study, self-catalytic maturation of heterologously expressed pro-galactose oxidase was analysed in E.coli by altering some amino acids which were supposed to play a crucial role in pro-peptide removal. Galactose oxidase (GOase; EC 1.1.3.9) from Fusarium graminearum; having a molecular mass of 68kDa, is a monomeric, copper containing enzyme with an unusual thioether bond. The enzyme is produced as a precursor with an additional 8 amino acid pre- and a 17- amino acid pro-sequence at the N terminus. Pr...
A comparative study on the phenolic composition, antioxidant and enzyme inhibition activities of two endemic Onosma species
Saravanakumar, Kandasamy; Sarikurkcu, Cengiz; Sarikurkcu, Rifat Tayyib; Wang, Myeong-Hyeon (Elsevier BV, 2019-12-15)
The studies on bioactivities of plant extracts are a fundamental requirement for future pharmacological research. Therefore, the present study extracted the phytochemicals from the two endemic species such as Onosma isaurica and O. bracteosa and tested their antioxidant, reducing power and enzyme inhibitory activities followed by scanning of the phytochemicals in the extracts by using liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS). The results revealed that the antioxidant activi...
SELECTION OF CULTURED WHEAT CELLS FOR TOLERANCE TO HIGH-TEMPERATURE STRESS
WANG, WC; SHANG, XM; YUCEL, M; NGUYEN, HT (Wiley, 1993-03-01)
In vitro culture offers approaches to study cellular responses and to obtain cells tolerant to high temperature stress. The objectives of this study were to select wheat cells that tolerate high temperature stress, study cell line stability and cytogenetic characteristics, and compare protein synthesis in selected and control cell lines after exposure to heat stress. Three of 600 colonies plated were recovered after cells had been exposed to 48-degrees-C. Forty-five percent of the cells of the tolerant cell...
Simultaneous dissolution and uptake of nutrients in microalgal treatment of the secondarily treated digestate
Ulgudur, Nilufer; Bayramoğlu, Tuba Hande; Demirer, Goksel N. (Elsevier BV, 2019-11-01)
Anaerobic digestion processes result in large volumes of digestates which still require the development of elaborate and viable management options to improve the sustainability of these processes. Coupling of secondary anaerobic treatment with a microalgal nutrient removal process may serve benefits such as residual biogas and associated greenhouse gas capture from the digestate content, microalgal biomass build-up and its further valorization. This study investigated the applicability a microalgal nutrient...
Citation Formats
M. Oezgen, M. Yildiz, N. Koyuncu, and S. Oende, “The effect of seed size on tissue culture response of callus from endosperm-supported mature embryos of barley (Hordeum vulgare L.),” CEREAL RESEARCH COMMUNICATIONS, pp. 1415–1425, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/67353.