Determination of Ochratoxin A in Black and White Pepper, Nutmeg, Spice Mix, Cocoa, and Drinking Chocolate by High-Performance Liquid Chromatography Coupled with Fluorescence Detection: Collaborative Study

Date

2017-09-01

Author

Cubero-Leon, Elena
Bouten, Katrien
Senyuva, Haviide
Stroka, Joerg

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

152
views

0
downloads

A method validation study for the determination of ochratoxin A in black and white pepper (Piper spp.), nutmeg (Myristica fragrans), spice mix (blend of ginger, turmeric, pepper, nutmeg, and chili), cocoa powder, and drinking chocolate was conducted according to the International Harmonized Protocol of the International Union of Pure and Applied Chemistry. The method is based on the extraction of samples with aqueous methanol, followed by a cleanup of the extract with an immunoaffinity column. The determination is carried out by reversed-phase LC coupled with a fluorescence detector. The study involved 25 participants representing a cross-section of research, private, and official control laboratories from 12 European Union (EU) Member States, together with Turkey and Macedonia. Mean recoveries ranged from 71 to 85% for spices and from 85 to 88% for cocoa and drinking chocolate. The RSDr values ranged from 5.6 to 16.7% for spices and from 4.5 to 18.7% for cocoa and drinking chocolate. The RSDR values ranged from 9.5 to 22.6% for spices and from 13.7 to 30.7% for cocoa and drinking chocolate. The resulting Horwitz ratios ranged from 0.4 to 1 for spices and from 0.6 to 1.4 for cocoa and drinking chocolate according to the Horwitz function modified by Thompson. The method showed acceptable within-laboratory and between-laboratory precision for each matrix, and it conforms to requirements set by current EU legislation.

Subject Keywords

Agronomy and Crop Science, Food Science, Analytical Chemistry, Pharmacology, Environmental Chemistry

URI

https://hdl.handle.net/11511/67356

Journal

JOURNAL OF AOAC INTERNATIONAL

DOI

https://doi.org/10.5740/jaoacint.16-0430

Collections

Unclassified, Article

Suggestions

OpenMETU
Core

Analysis of Deoxynivalenol, Zearalenone, T-2, and HT-2 Toxins in Animal Feed by LC/MS/MS-A Critical Comparison of Immunoaffinity Column Cleanup with No Cleanup Senyuva, Hamide Z.; Gilbert, John; Turkoz, Gozde; Leeman, David; Donnelly, Carol (Oxford University Press (OUP), 2012-11-01) A comparison has been made of an LC/MS/MS method using direct analysis of acetonitrile extracts of feed and cereal samples and a method using acetonitrile extraction and subsequent immunoaffinity column (IAC) cleanup. Naturally contaminated samples containing one or more of deoxynivalenol, zearalenone, T-2, and HT-2 toxins were analyzed together with test materials containing known toxin levels. LC/MS/MS ion ratios and peak profiles, repeatability, and LOQs were used as the basis for comparing the two appro...
Differentiation of Anatolian honey samples from different botanical origins by ATR-FTIR spectroscopy using multivariate analysis Gok, Seher; Severcan, Mete; Goormaghtigh, Erik; KANDEMİR, İRFAN; Severcan, Feride (Elsevier BV, 2015-03-01) Botanical origin of the nectar predominantly affects the chemical composition of honey. Analytical techniques used for reliable honey authentication are mostly time consuming and expensive. Additionally, they cannot provide 100% efficiency in accurate authentication. Therefore, alternatives for the determination of floral origin of honey need to be developed. This study aims to discriminate characteristic Anatolian honey samples from different botanical origins based on the differences in their molecular co...
DETERMINATION OF SELENIUM IN HUMAN DIETS BY RADIOCHEMICAL NEUTRON-ACTIVATION ANALYSIS ELHALLAQ, YH; GOKMEN, IG; ARAS, NK; Gökmen, Ali (Royal Society of Chemistry (RSC), 1992-03-01) A post-irradiation radiochemical separation technique was tested for the determination of selenium levels in diet samples, collected by using a duplicate portion technique, from both rural and urban population groups in Turkey. The technique involved sample irradiation, acid digestion, selective distillation, precipitation and filtration steps. During the separations it was possible to determine the yield of each sample using a stable selenium carrier. An average chemical yield of 71 +/- 3% was obtained ...
Production of hazelnut skin fibres and utilisation in a model bakery product Cikrikci, SEVİL; Demirkesen, I.; Mert, Behiç (Codon Publications, 2016-01-01) Microfluidisation was used as a potential method for the production of fibrous structure from hazelnut skin (HS), and which was evaluated as an ingredient in cake as model bakery product. More specifically, effects of different amounts of microfluidised HS on rheological behaviour of cake batter and quality parameters (texture and colour) and storage characteristics of cake products were evaluated. Furthermore, performance of microfluidised HS was compared with conventionally milled HS and cacao powder for ...
Correlation between physical and sensorial properties of gummy confections with different formulations during storage Tireki, Suzan; Şümnü, Servet Gülüm; Şahin, Serpil (Springer Science and Business Media LLC, 2021-01-01) Gummy confections are popular products formulated with sucrose, glucose syrups, gelling agents, acids, flavourings and colouring agents. They have various commercial types in terms of formulation, texture, taste and colour, however, there is inadequate investigation on storage behavior of these products. The aim of this study is to investigate the effects of glucose syrup:sucrose ratio (1.1 and 1.5), starch (0% and 1.5%) and gelatine concentration (3% and 6%) on texture profile, colour and sensory propertie...

Citation Formats

E. Cubero-Leon, K. Bouten, H. Senyuva, and J. Stroka, “Determination of Ochratoxin A in Black and White Pepper, Nutmeg, Spice Mix, Cocoa, and Drinking Chocolate by High-Performance Liquid Chromatography Coupled with Fluorescence Detection: Collaborative Study,” JOURNAL OF AOAC INTERNATIONAL, pp. 1458–1468, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/67356.