DETERMINATION OF SELENIUM IN HUMAN DIETS BY RADIOCHEMICAL NEUTRON-ACTIVATION ANALYSIS

Date

1992-03-01

Author

ELHALLAQ, YH
GOKMEN, IG
ARAS, NK
Gökmen, Ali

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

215
views

0
downloads

A post-irradiation radiochemical separation technique was tested for the determination of selenium levels in diet samples, collected by using a duplicate portion technique, from both rural and urban population groups in Turkey. The technique involved sample irradiation, acid digestion, selective distillation, precipitation and filtration steps. During the separations it was possible to determine the yield of each sample using a stable selenium carrier. An average chemical yield of 71 +/- 3% was obtained for the radiochemical neutron activation analysis. For samples from urban and rural regions, the average selenium concentrations obtained were 0.14 +/- 0.04 and 0.07 +/- 0.02 mg kg-1, respectively. It was also possible to determine daily dietary selenium intakes, which were found to be 81 +/- 41-mu-g and 23 +/- 11-mu-g for the urban and rural groups, respectively. Although daily selenium intakes were found for a small number of subjects in this study, the separation technique developed can be used for determination of the selenium status in larger population groups.

Subject Keywords

Analytical Chemistry, Spectroscopy, Electrochemistry, Biochemistry, Environmental Chemistry

URI

https://hdl.handle.net/11511/63304

Journal

ANALYST

DOI

https://doi.org/10.1039/an9921700447

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

DETERMINATION OF SELENIUM IN BIOLOGICAL MATRICES USING A KINETIC CATALYTIC METHOD GOKMEN, IG; ABDELQADER, E (Royal Society of Chemistry (RSC), 1994-04-01) A simple and sensitive catalytic spectrophotometric method was developed for the determination of selenium in biological matrices. The method is based on the catalytic effect of selenium on the reaction of Methylene Blue (MB) with sodium sulfide. For a given reaction between MB and sodium sulfide, the change in the MB absorbance with time was monitored, then the time (t) required for completion of the reaction was determined, and t(-1) was calculated. A plot of t(-1) versus selenium concentration constitute...
A rapid method for detection of genetically modified organisms based on magnetic separation and surface-enhanced Raman scattering GÜVEN, BURCU; BOYACI, İSMAİL HAKKI; TAMER, UĞUR; Çalık, Pınar (Royal Society of Chemistry (RSC), 2012-01-01) In this study, a new method combining magnetic separation (MS) and surface-enhanced Raman scattering (SERS) was developed to detect genetically modified organisms (GMOs). An oligonucleotide probe which is specific for 35 S DNA target was immobilized onto gold coated magnetic nanospheres to form oligonucleotide-coated nanoparticles. A self assembled monolayer was formed on gold nanorods using 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB) and the second probe of the 35 S DNA target was immobilized on the activa...
2-DIMENSIONAL ELECTROPHORESIS OF PROTEINS WITH A DIFFERENT APPROACH TO ISOELECTRIC-FOCUSING NAQVI, SMS; OZALP, VC; Öktem, Hüseyin Avni; YUCEL, M (Royal Society of Chemistry (RSC), 1994-06-01) A simple method for the two-dimensional analysis of proteins was developed based on direct extraction of a protein sample from dried/precipitated material into isoelectric focusing (IEF) gel solution. This sample solution is equally useful for sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis and two-dimensional electrophoresis. For the former method this sample solution is applied directly to the gel. For the tatter, sample solution containing a desired amount of proteins is mixed with IEF ge...
Determination of esterase enzyme polymorphism in house fly (Musca domestica L.) populations in Turkey TAŞKIN, VATAN; Taskin, Belgin Gocmen; KÜÇÜKAKYÜZ, KÖKSAL; Kence, Meral (2011-01-01) The objective of this study was to determine esterase enzyme polymorphism in 16 natural Musca domestica L. populations from the Aegean and Mediterranean regions of Turkey by using the native polyacrylamide gel electrophoresis (PAGE) technique. The high level of genetic variation among populations and species make esterase enzymes an important tool for analyzing genetic differentiation and evolutionary relationships in insects. In the present study, 22 a-esterase bands were detected by using a-naphthylacetat...
FLUORESCENT COUMARIN COMPONENTS OF THE BARK OF AESCULUS HIPPOCASTANUM Çoruh, Nursen (Informa UK Limited, 2014-06-15) HPLC isolation and spectroscopic characterization studies, along with the examination of DPPH radical scavenging capacity, were performed for identifying the major coumarin constituents of the bark extract of Aesculus hippocastanum L. collected from Turkey. The isolation process was carried out using a semi-preparative RP-HPLC with diode array detector. The major phenolic components of bark extract were identified by means of analytic RP-HPLC. Isolated compounds were characterized when necessary, along with...

Citation Formats

Y. ELHALLAQ, I. GOKMEN, N. ARAS, and A. Gökmen, “DETERMINATION OF SELENIUM IN HUMAN DIETS BY RADIOCHEMICAL NEUTRON-ACTIVATION ANALYSIS,” ANALYST, pp. 447–451, 1992, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/63304.