A critical review of the specifications and performance of antibody and DNA-based methods for detection and quantification of allergens in foods

Senyuva, Hamide Z.
Jones, Ivona Baricevic
Sykes, Mark
Baumgartner, Sabine
Despite the availability of a large number of antibody and DNA based methods for detection and quantification of allergens in food there remain significant difficulties in selecting the optimum technique to employ. Published methods from research groups mostly contain sufficient detail concerning target antigen, calibration procedures and method performance to allow replication by others. However, routine allergen testing by the food industry relies upon commercialised test kits and frequently the suppliers provide disappointingly little specification detail on the grounds that this is proprietary information. In this review we have made a critical assessment of the published literature describing the performance of both commercial and non-commercial test kits for food allergens over the period 2008-2018. Mass spectrometric methods, which have the potential to become reference methods for allergens, are not covered in this review. Available information on the specifications of commercial ELISA and LFD test kits are tabulated for milk, egg and peanut allergens, where possible linking to publications concerning collaborative studies and proficiency testing. For a number of commercial PCR test kits, specifications provided by manufacturers for detection of a small selection of allergen are tabulated. In conclusion we support the views of others of the critical need for allergen reference materials as the way forward to improve the comparability of different testing strategies in foods.


Analysis and critical comparison of food allergen recalls from the European Union, USA, Canada, Hong Kong, Australia and New Zealand
Bucchini, Luca; Guzzon, Antonella; Poms, Roland; Senyuva, Hamide (Informa UK Limited, 2016-01-01)
As part of a European Union-funded project (FP7) developing 'Integrated approaches to food allergen and allergy management', a database was constructed based on publicly available information on food allergen recalls in Europe, North America, Hong Kong, Australia and New Zealand. Over 2000 entries were made into the database. The database covers a 4-year period from 2011 to 2014 and each entry is categorised into food type (two different classifications), identified allergen and cause where indicated by the...
Rapid LC and LC/MS for routine analysis of mycotoxins in foods
Senyuva, H.; Gilbert, J.; Özcan Kabasakal, Süreyya; Gurel, N. (Wageningen Academic Publishers, 2008-08-01)
Affinity column clean-up of food samples for mycotoxin analysis produces extracts which are free of co-extractives and therefore require little chromatography for separation and quantification of the target analytes. Using such clean extracts, we report rapid chromatographic methods for aflatoxins B(1), B(2), G(1) and G(2), aflatoxin M(1), ochratoxin A, zearalenone and fumonisins. Using short columns with small particle size packing, HPLC conditions have been developed reducing analysis time typically by 75...
Future perspectives in Orbitrap (TM)-high-resolution mass spectrometry in food analysis: a review
Senyuva, Hamide Z.; GÖKMEN, VURAL; Sarikaya, Ebru Ates (Informa UK Limited, 2015-10-03)
A literature search from 2007 to 2014 was conducted to identify publications where principally LC-Orbitrap-high-resolution mass spectrometry (HRMS) has been employed in food analysis. Of a total of 212 relevant references, only 22 papers were from 2007-10, but in subsequent years there has been a steady growth in publications with 38-55 relevant papers being published each year from 2011 to 2014. In the food safety area, over 50% of the published papers were equally divided between pesticides, veterinary dr...
Occurrence of fungi and their mycotoxins in individual Turkish dried figs
Senyuva, H. Z.; Gilbert, J.; Samson, R. A.; Özcan Kabasakal, Süreyya; Ozturkoglu, S.; Onal, D. (Wageningen Academic Publishers, 2008-02-01)
Fifty individual figs which had been rejected as potentially contaminated by sorting under UV light were separately analysed to identify the presence of fungi and their mycotoxins. Aflatoxin B-1 was found in 49 samples with levels ranging from 0.7 to 222 ng g(-1), with 40 individual figs containing more than 2 ng g(-1), indicating the efficacy of the UV screening process in identifying contaminated fruit. Ochratoxin A (OTA) was found in 32 of the figs at levels from 0.4 to 1710 ng g(-1), with 50% of the sam...
The growth behavior of Chlorella vulgaris in the presence of 4-chlorophenol and 2,4-dichlorophenol
ŞAHİNKAYA, Erkan; Dilek, Filiz Bengü (Elsevier BV, 2009-03-01)
Toxicity of 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) on the growth of Chlorella vulgaris was investigated in batch reactors. Results revealed that 4-CP did not adversely affect the growth of algae up to 20 mg/L, however higher concentrations inhibited growth appreciably and no growth was detected at 100 mg/L. 4-CP also caused some physiological changes in the algal cells as increasing initial 4-CP concentration caused a linear decrease in chlorophyll a (chl-a) content of the cell. 2,4-DCP up t...
Citation Formats
H. Z. Senyuva, I. B. Jones, M. Sykes, and S. Baumgartner, “A critical review of the specifications and performance of antibody and DNA-based methods for detection and quantification of allergens in foods,” FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, pp. 507–547, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/67467.