Rapid LC and LC/MS for routine analysis of mycotoxins in foods

2008-08-01
Senyuva, H.
Gilbert, J.
Özcan Kabasakal, Süreyya
Gurel, N.
Show full item record
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
132
views
0
downloads
Affinity column clean-up of food samples for mycotoxin analysis produces extracts which are free of co-extractives and therefore require little chromatography for separation and quantification of the target analytes. Using such clean extracts, we report rapid chromatographic methods for aflatoxins B(1), B(2), G(1) and G(2), aflatoxin M(1), ochratoxin A, zearalenone and fumonisins. Using short columns with small particle size packing, HPLC conditions have been developed reducing analysis time typically by 75%, e.g. for aflatoxins from 19.0 mm to 4.0 min with full separation of the four toxins and for aflatoxin M(1) giving an analysis time of less than 1 min compared to 5 min for conventional analysis. Fumonisins were analysed directly by LC/MS in a run time of 4.1 min, using selected ion monitoring to avoid the need for derivatisation for fluorescence detection by HPLC. Peak purity under rapid analysis conditions has been demonstrated by LC/MS for a variety of food extracts such as pistachios, hazelnuts, paprika, cocoa, coffee, cereals, sesame oil, cheese, maize, animal feed, dried figs, dried vine fruits, cornflakes and bread. These substantial reductions in analysis time offer significant benefits to laboratories undertaking routine screening and quantitative analysis of mycotoxins in foods.
Toxicology, Food Science, Public Health, Environmental and Occupational Health
https://hdl.handle.net/11511/45976
WORLD MYCOTOXIN JOURNAL
Department of Chemistry, Article

Suggestions

Trace level determination of beryllium in natural and flavored mineral waters after pre-concentration using activated carbon
KILINÇ, ERSİN; Bakirdere, Sezgin; YAMAN, MEHMET (Informa UK Limited, 2011-01-01)
The concentrations of beryllium (Be) in natural and flavored mineral water samples were determined by flame atomic absorption spectrophotometer (FAAS) after pre-concentration based on the complexation of Be(+2) with a mixture of acetylacetone (pentane-2,4-dione) plus morin (3,5,7,2',4'-pentaoxyflavone) and adsorption on activated carbon. The adsorbed complex was eluted with 1.5 ml of 2.0 M HNO(3) and evaporated to dryness. After adding 1.5 ml of 2 M HNO(3) and centrifuging, Be in acid solution was determine...
A critical review of the specifications and performance of antibody and DNA-based methods for detection and quantification of allergens in foods
Senyuva, Hamide Z.; Jones, Ivona Baricevic; Sykes, Mark; Baumgartner, Sabine (Informa UK Limited, 2019-01-01)
Despite the availability of a large number of antibody and DNA based methods for detection and quantification of allergens in food there remain significant difficulties in selecting the optimum technique to employ. Published methods from research groups mostly contain sufficient detail concerning target antigen, calibration procedures and method performance to allow replication by others. However, routine allergen testing by the food industry relies upon commercialised test kits and frequently the suppliers...
Analysis of Deoxynivalenol, Zearalenone, T-2, and HT-2 Toxins in Animal Feed by LC/MS/MS-A Critical Comparison of Immunoaffinity Column Cleanup with No Cleanup
Senyuva, Hamide Z.; Gilbert, John; Turkoz, Gozde; Leeman, David; Donnelly, Carol (Oxford University Press (OUP), 2012-11-01)
A comparison has been made of an LC/MS/MS method using direct analysis of acetonitrile extracts of feed and cereal samples and a method using acetonitrile extraction and subsequent immunoaffinity column (IAC) cleanup. Naturally contaminated samples containing one or more of deoxynivalenol, zearalenone, T-2, and HT-2 toxins were analyzed together with test materials containing known toxin levels. LC/MS/MS ion ratios and peak profiles, repeatability, and LOQs were used as the basis for comparing the two appro...
Fatty acid methyl ester analysis of bacterşal isolates from salt lake, Turkey and characterization of their extracellular enzymes
Bahçeci, Hümeyra; Gültekin, Güzin Candan; Department of Biotechnology (2004)
In this study, 11 bacterial isolates from Salt Lake,Turkey were identified by using fatty acid methyl ester (FAME) analysis. They were screened for production of industrially important enzymes xylanase, cellulase, a-amylase and protease. These enzymes were characterized in terms of enzyme activity, stability, optimum temperature and optimum pH. One of the isolates was identified as Bacillus pumilus, and two of them were identified as Bacillus subtilis. Other isolates were determined to be Bacillus lichenifo...
SODIUM, RUBIDIUM AND CESIUM UPTAKE ON LOS-TRANCOS CLAY
GOKTURK, H; DYER, A (Springer Science and Business Media LLC, 1994-10-03)
The uptake of sodium, caesium and rubidium onto Los Trances clay was studied using the batch method. Sodium-22, rubidium-86 and caesium-137 were used as tracers. In most of the experiments cation concentrations used were 0.01 N. Triple distilled and deionized water ware used and the grain size of all solid particles was less than or equal to 53 mu m. In all cases a few hours of shaking was enough to reach the equilibrium. The ion exchange isotherm experiments were performed by using ammonium forms of the cl...
Citation Formats
H. Senyuva, J. Gilbert, S. Özcan Kabasakal, and N. Gurel, “Rapid LC and LC/MS for routine analysis of mycotoxins in foods,” WORLD MYCOTOXIN JOURNAL, pp. 229–235, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/45976.
METU IIS - Integrated Information Service open@metu.edu.tr