Effects of fetal alcohol and maternal intubation stress on the expression of proteins controlling postnatal development of male rat hippocampus

Date

2020-09-01

Author

ELİBOL, BİRSEN
BEKER, MERVE
Jakubowska-Dogru, Ewa
KILIÇ, ÜLKAN

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

186
views

0
downloads

Background Developing brains can partially get over prenatal alcohol exposure-related detrimental conditions by activating some mechanisms involved in survival. Objectives This study aimed to shed light on the molecular correlates of compensatory mechanisms by examining temporal profiles in the expression of proteins controlling postnatal development in the rat hippocampus prenatally exposed to intubation stress/ethanol. Methods Male pups were randomly assigned to age subgroups (n = 21/age) which were sacrificed on postnatal day (PD)1, PD10, PD30, and PD60. Ethanol (6 g/kg/day) were intragastrically intubated to the dams throughout 7-21 gestation days. The expression of neurogenesis and angiogenesis markers, extracellular matrix proteins, and growth-promoting ligands were examined by western blot. Results The most rapid increase in the index of neuronal maturation was noted between PD10-PD30 (p< .05). Prenatal stress caused a decrease of neurogenesis markers at birth and an increase of their expressions at PD10 and PD30 to reach control levels (p< .001). The impact of fetal-alcohol was observed as a decrease in the expression of synaptic plasticity protein versican at birth (p< .001), an increase in the synaptic repulsion protein ephrin-B2 at PD10 (p< .001), and a decrease in the maturation of BDNF at PD30 (p< .001) with a decrease in the mature neuron markers at PD30 (p< .001) and PD60 (p= .005) which were compensated with upregulation of angiogenesis and increasing brevican expression, a neuronal maturation protein (p< .001). Conclusion These data providein vivoevidence for the potential therapeutic factors related to neurogenesis, angiogenesis, and neurite remodeling which may tolerate the alcohol/stress dependent teratogenicity in the developing hippocampus.

Subject Keywords

Medicine (miscellaneous), Clinical Psychology, Psychiatry and Mental health

URI

https://hdl.handle.net/11511/67855

Journal

AMERICAN JOURNAL OF DRUG AND ALCOHOL ABUSE

DOI

https://doi.org/10.1080/00952990.2020.1780601

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Effects of Early Postnatal Exposure to Ethanol on Retinal Ganglion Cell Morphology and Numbers of Neurons in the Dorsolateral Geniculate in Mice Dursun, Ilknur; Jakubowska-Dogru, Ewa; van der List, Deborah; Liets, Lauren C.; Coombs, Julie L.; Berman, Robert F. (Wiley, 2011-11-01) Background: The adverse effects of fetal and early postnatal ethanol intoxication on peripheral organs and the central nervous system are well documented. Ocular defects have also been reported in about 90% of children with fetal alcohol syndrome, including microphthalmia, loss of neurons in the retinal ganglion cell (RGC) layer, optic nerve hypoplasia, and dysmyelination. However, little is known about perinatal ethanol effects on retinal cell morphology. Examination of the potential toxic effects of alcoh...
The Effects of Short-Term Chronic Ethanol Intoxication and Ethanol Withdrawal on the Molecular Composition of the Rat Hippocampus by FT-IR Spectroscopy Elibol-Can, Birsen; Jakubowska-Dogru, Ewa; Severcan, Mete; Severcan, Feride (Wiley, 2011-11-01) Background: The numerous adverse effects of ethanol abuse and ethanol withdrawal on biological systems are well documented. Conversely, the understanding of the molecular mechanisms underlying these pathological effects is still incomplete. This study was undertaken to investigate the effects of short-term chronic ethanol administration and ethanol withdrawal on the molecular structure and function of hippocampal tissue, a brain region important for mnemonic processes and known to be highly susceptible to e...
Factors associated with the symptoms of depression and anxiety among male Turkish prisoners: a life crisis and personal growth model perspective ŞENOL DURAK, EMRE; Gençöz, Faruk (Informa UK Limited, 2010-01-01) The factors associated with depression and anxiety disorders in the prison populations have been empirically tested in various studies. The aim of the present study was to examine the factors associated with the symptoms of depression and anxiety in a sample of 179 Turkish male prisoners. For this purpose, a model conceptualized by Schaefer and Moos (1998) was used as the theoretical framework for this study. The role of locus of control (as a personality trait); prison type, crime type, time spent in priso...
Patterns of Depression in Medical Patients and Their Relationship with Causal Attrihutions for Illness Karanci, Nuray A. (S. Karger AG, 1988) he present study investigated the factor structure of the Beck Depression Inventory (BDI), and causal attributions for the development of illness in a sample of 102 inpatients of a thoracic surgery department, with the main objective of examining the power of causal attributions and functional support in predicting different factors derived from the BDI. The results revealed that the BDI clusters into affective/motivational, somatic/vegetative, self-blame and self-punitiveness dimensions. Causal attribution...
Effects of early postnatal alcohol exposure on the developing retinogeniculate projections in C57BL/6 mice DURSUN, İLKNUR; Jakubowska-Dogru, Ewa; Elibol-Can, Birsen; van der List, Deborah; Chapman, Barbara; Qi, Lihong; Berman, Robert F. (Elsevier BV, 2013-05-01) Previous studies on the adverse effects of perinatal exposure to ethanol (EtOH) on the developing visual system mainly focused on retinal and optic nerve morphology. The aim of the present study was to investigate whether earlier reported retinal and optic nerve changes are accompanied by anomalies in eye-specific fiber segregation in the dorsal lateral geniculate nucleus (dLGN). C57BL/6 mice pups were exposed to ethanol by intragastric intubation at either 3 or 4 g/kg from postnatal days (PD) 3-10, the thi...

Citation Formats

B. ELİBOL, M. BEKER, E. Jakubowska-Dogru, and Ü. KILIÇ, “Effects of fetal alcohol and maternal intubation stress on the expression of proteins controlling postnatal development of male rat hippocampus,” AMERICAN JOURNAL OF DRUG AND ALCOHOL ABUSE, pp. 0–0, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/67855.