Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Cytotoxic effects of four different endodontic materials in human periodontal ligament fibroblasts
Date
2007-12-01
Author
GÖRDUYSUS, MELAHAT
AVCU, NİHAL
Gorduysus, Omer
Pekel, Aysel
Baran, Yusuf
Avcu, Ferit
Ural, Ali Ugur
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
212
views
0
downloads
Cite This
The purpose of this study was to compare the cytotoxicity, induced apoptosis and/or necrosis, and apoptotic mechanisms in human periodontal ligament (PDL) fibroblasts treated with four different endodontic materials: White ProRoot mineral trioxide aggregate (MTA) (MTA/Dentsply; Tulsa Dental, Memphis, TN), Diaket (ESPE, Seefeld, Germany), Endion (VOCO, Cuxhaven, Germany), and CYMED 8410 (NANO, Kaohsiung, Taiwan). The effects of these four materials on the viability of PDL fibroblasts were determined by MTT (3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-SH-tetrazolium bromide) assay. Apoptotic pathways were evaluated via several mechanisms. Exposure to MTA for 24, 48, and 72 hours resulted in no significant differences in MTT reduction and viable cell number compared with controls. However, treatment of PDL fibroblasts with Diaket Endion, and CYMED 8410 for 24, 48, and 72 hours resulted in cytotoxicity with MTT and a reduction of viable cell number with trypan blue dye exclusion test compared with controls (from p < 0.05 to p < 0.001). Annexin V-FITC/PI staining showed that Diaket, Endion, and CYMED 8410 induced higher percentages of apoptosis and/or necrosis than in controls (45.6%, 25.5%, and 6.3%, respectively). Results of cell-cycle analyses were concordant with annexin V-FITC/PI staining findings. These results suggest that MTA is a very biocompatible filling material. However, Diaket, Endion, and CYMED 8410 are toxic to PDL fibroblasts in vitro. The main form of cell death induced by these filling materials was determined to be apoptosis and/or necrosis.
Subject Keywords
General Dentistry
URI
https://hdl.handle.net/11511/68407
Journal
JOURNAL OF ENDODONTICS
DOI
https://doi.org/10.1016/j.joen.2007.08.017
Collections
Department of Biology, Article
Suggestions
OpenMETU
Core
ANALYSIS OF STRESS-DISTRIBUTION IN A VERTICALLY CONDENSED MAXILLARY CENTRAL INCISOR ROOT-CANAL
YAMAN, SD; ALACAM, T; Yaman, Yavuz (Elsevier BV, 1995-06-01)
In this study, the stress distribution in a maxillary central incisor subjected to an endodontic treatment was determined by using a three-dimensional finite-element method, The tooth had a variable root canal cross-sectional area, and the canal was assumed to be filled with vertical condensation. The Clinical practice was simulated by considering the obturation in successive stages, The main aim of the study was to investigate the possibility of fracture due to the obturation process, It was found that, wi...
The effect of acquired salivary pellicle on the surface free energy and wettability of different denture base materials
Sipahi, C; Anil, N; Bayramlı, Erdal (Elsevier BV, 2001-03-01)
Objective: The objective of the present study was to evaluate the effect of salivary coating on the wettability and surface free energy of different denture base materials.
The biomechanical relationship between incisor and condylar guidances in deep bite and normal cases
Darendeliler, N; Dincer, M; Soylu, Reşit (Wiley, 2004-05-01)
The purpose of this study was to investigate the biomechanical relationship between the condylar and anterior guidances in deep bite malocclusion and control groups. The subjects consisted of 18 deep bite patients and 14 normal occlusions. A four-bar mechanism was described on the lateral cephalogram films and a computer program, for which the lengths of the links measured on the films were the inputs, was developed. The computer program was used to calculate the angles of rotation of the mandible and the c...
In vitro Comparison of Autoclave Polymerization on the Transverse Strength of Denture Base Resins
Durkan, Rukiye; ÖZEL, MEHMET BİROL; Bagis, Bora; Usanmaz, Ali (Japanese Society for Dental Materials and Devices, 2008-07-01)
The aim of this study was to determine the effect of autoclave polymerization on the transverse strength of denture base polymers. To this end, 30 rectangular test specimens were fabricated of two heat-polymerized denture base polymers. The test groups were: (I) control. i.e.. conventional water bath to polymerize result by heat at 100 degrees C for 30 minutes; (II) autoclave polymerization at 60 degrees C for 30 minutes followed by 130 degrees C for 10 minutes; and (III) autoclave polymerization at 60 degr...
Finite element analysis of strength characteristics of various resin based restorative materials in Class V cavities
Yaman, SD; Şahin, Melin; Aydin, C (Wiley, 2003-06-01)
This study investigated the strength characteristics of various composites (Tetric Ceram, Tetric Flow, Prisma AP.H, Synergy Duo Shade, Synergy Compact, and Z 100) and compomers (Compoglass, Compoglass F, Dyract AP, F 2000) that were applied in a Class V cavity of a maxillary central incisor. The study was conducted by using a 3-dimensional finite element approach and in the study ansys package program was used. The tooth model had 294 elements and 420 nodes. The teeth considered were assumed to be subjected...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
M. GÖRDUYSUS et al., “Cytotoxic effects of four different endodontic materials in human periodontal ligament fibroblasts,”
JOURNAL OF ENDODONTICS
, pp. 1450–1454, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/68407.