Determination of Arsenobetaine in Fish Tissue by Species Specific Isotope Dilution LC-LTQ-Orbitrap-MS and Standard Addition LC-ICPMS

Date

2011-05-01

Author

Yang, Lu
Ding, Jianfu
Maxwell, Paulette
McCooeye, Margaret
Windust, Anthony
Ouerdane, Laurent
Bakirdere, Sezgin
Willie, Scott
Mester, Zoltan

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

229
views

0
downloads

An accurate and precise method for the determination of arsenobetaine (AsB, (CH(3))(3)(+)AsCH(2)COO(-)) in fish samples using exact matching species specific isotope dilution (ID) liquid chromatography LTQ:Orbitrap mass spectrometry (LC-LTQ-Orbitrap-MS) and standard addition LC inductively coupled plasma mass spectrometry (LC-ICPMS) is described. Samples were extracted by sonication for 30 min with high purity deionized water. An in-house synthesized (13)C enriched AsB spike was used for species specific ID analysis whereas natural abundance AsB, synthesized and characterized by quantitative (1)H NMR (nuclear magnetic resonance, spectroscopy), was used for reverse ID and standard addition LC-ICPMS. With the LTQ-Orbitrap-MS instrument in scan mode (m/z 170-190) and resolution set at 7500, the intensities of [M + H](+) ions at m/z of 179.0053 and 180.0087 were used to calculate the 179.0053/180.0087 ion ratio for quantification of AsB in fish tissues. To circumvent potential difficulty in mass bias correction, an exact matching approach was applied. A quantitatively prepared mixture of the natural abundance AsB standard and the enriched spike to give a ratio near one was used for mass bias correction. Concentrations of 9.65 +/- 0.24 and 11.39 +/- 0.39 mg kg(-1) (expanded uncertainty, k = 2) for AsB in two fish samples of fish1 and fish2, respectively, were obtained by ID LC-LTQ-Orbitrap-MS. These results are in good agreement with those obtained by standard addition LC-ICPMS, 9.56 +/- 0.32 and 11.26 +/- 0.44 mg kg(-1) (expanded uncertainty, k = 2), respectively. Fish CRM DORM-2 was used for method validation and measured results of 37.9 +/- 1.8 and 38.7 +/- 0.66 mg kg(-1) (expanded uncertainty, k = 2) for AsB obtained by standard addition LC-ICPMS and ID LC-LTQ-Orbitrap-MS, respectively, are in good agreement with the certified value of 39.0 +/- 2.6 mg kg(-1) (expanded uncertainty, k = 2). Detection limits of 0.011 and 0.033 mg kg(-1) for AsB with LC-ICPMS and ID LC-LTQ-Orbitrap-MS, respectively, were obtained demonstrating that the technique is well suited to the determination AsB in fish samples. To the best of our knowledge, this is first application of species specific isotope dilution for the accurate and precise determination of AsB in biological tissues.

Subject Keywords

Analytical Chemistry

URI

https://hdl.handle.net/11511/68527

Journal

ANALYTICAL CHEMISTRY

DOI

https://doi.org/10.1021/ac103258m

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Determination of germanium at trace levels by chloride generation atomic absorption spectrometry Kaya, Murat; Volkan, Mürvet; Department of Chemistry (2004) Trace amounts of germanium is determined by flame atomic absorption spectrometry by utilizing the vaporization of germanium tetrachloride. Using a continuous flow reactor, sample solution is mixed with concentrated hydrochloric acid and heated to 80ð C to form volatile germanium tetrachloride which can be subsequently sent to N2O-C2H2 flame AAS. The necessary conditions for the volatilization of germanium tetrachloride are investigated in detail and the applicability of the method for the determination of t...
Determination of silver by chemical vapour generation and atomic absoption spectrometry Öztürk, Çağla Pınar; Ataman, Osman Yavuz; Department of Chemistry (2004) A method for determination of silver has been developed based on chemical vapour generation atomic absorption spectrometry (CVGAAS). Volatile species of silver in acidified medium were generated by the reduction of sodium tetrahydroborate; these species were sent to a flame-heated quartz tube atomizer (QTA) following isolation by using a gas-liquid separator. Flow injection (FI) was used for sample introduction. Optimization of parameters such as; concentrations of acid and NaBH4 concentration, flow rates o...
Determination of cadmium using slotted quartz tube atom trap atomic absorption spectrometry and metal coatings Özcan Gurbetçioğlu, G. Pelin; Ataman, Osman Yavuz; Department of Chemistry (2010) Flame atomic absorption spectroscopy (FAAS) is a common technique for detecting metals and metalloids in environmental, biological and metallurgical samples. Although it is a rather old technique, it is still very reliable, simple to use and inexpensive. The technique can be used to determine the concentration of over 70 different metals in a solution. However, it has detection limits at mg/L levels. Some atom trapping methods have been developed to reach the detection limits of ng/mL levels. Slotted quartz...
Use of multi-walled carbon nanotubes in matrix solid phase dispersion extraction combined with gas chromatography Njie, Njaw; Aygün, Rüveyde Sezer; Department of Chemistry (2008) The use of Multi-Walled Carbon Nanotubes (MWCNT) as solid sorbent in Matrix Solid-Phase Dispersion (MSPD) extraction and preconcentration method was presented to determine some commonly used organophosphorus insecticides/OPIs in honey samples using a Gas Chromatography Flame Ionization Detector (GC-FID). OPIs are poisonous compounds used to kill insects and rodents by affecting their nervous system. The limit of detections obtained after MSPD extraction were 7.0 ng/g for Malathion, Malaoxon and Fenitrothion...
PRECONCENTRATION OF SOME TRACE-METALS FROM SEA-WATER ON A MERCAPTO-MODIFIED SILICA-GEL Volkan, Mürvet; Ataman, Osman Yavuz (1987-10-01) Silica modified by the addition of mercapto chelating groups has been developed for the pre-concentration of trace metals from natural waters. This material removes cadmium, copper, lead and zinc from aqueous solution and can be employed for the pre-concentration of these metals by both column and batch techniques. Under column and batch conditions recoveries larger than 95% were common. For batch extractions of cadmium, zinc, copper and lead from sea water, recoveries of 91 ± 5, 98 ± 4, 97 ± 4 and 96 ± 5%,...

Citation Formats

L. Yang et al., “Determination of Arsenobetaine in Fish Tissue by Species Specific Isotope Dilution LC-LTQ-Orbitrap-MS and Standard Addition LC-ICPMS,” ANALYTICAL CHEMISTRY, pp. 3371–3378, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/68527.