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Bacillus thuringiensis alttür morrisoni Kitinaz Geninin Karakterizasyonu
Date
2007-10-28
Author
Özcengiz, Gülay
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https://hdl.handle.net/11511/75273
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Bacillus subtilis dipeptid sentataz geninin klonlanması ve ifade edilmesi
Karataş, Ayten Yazgan; Özcengiz, Gülay; Alaeddinoğlu, Gürdal N.(1999)
Bu çalışmada, Bacillus subtilis 168 (PY79) tarafından nonribosomal olarak sentezlenen, L-alanin ve L-anticapsin'den oluşan dipeptid bir antibiyotik olan bacilysinin biyosentezinden sorumlu gen ve gen kümelerini belirlemek ve karakterize edebilmek için klonlama çalışmaları gerçekleştirilmiştir. Mini-Tn10 taşıyan pIC333 vektörü ile "Tn10 insertion mutation" metodu uygulanmış ve gayet başardı sonuçlar elde edilmiştir. Bu metod sonucunda bacilycin üretemeyen ve $KA_1, KA_2, KA_3 ve KA_4$ olarak isimlendirilen d...
Proteome-wide analysis of the functional roles of Bacilysin biosynthesis in Bacillus subtilis
Taşkın Aras, Aslı; Özcengiz, Gülay; Department of Biology (2010)
The members of the genus Bacillus produce a wide variety of secondary metabolites with antimetabolic and pharmacological activities. Most of these metabolites are small peptides that have unusual components and chemical bonds and synthesized nonribosomally by multifunctional enzyme complexes called peptide synthetases. Bacilysin, being produced and excreted by certain strains of Bacillus subtilis, is one of the simplest peptide antibiotics known. It is a dipeptide with an N-terminal L-alanine and an unusual...
Comparative transcriptomics of bacillus subtilisbacilysin-negative genotype with particular reference to bacilysin formation
Kocabay, Naz; Özcengiz, Gülay; Department of Molecular Biology and Genetics (2021-2-15)
The Gram-positive model prokaryote Bacillus subtilisis a non-pathogenic organism that is known for producing a wide variety of secondary metabolites with pharmacological andantimicrobial activities.The smallestdipeptideantibiotic bacilysin is among the many secondary metabolites that are produced by B. subtilis, and it is composed of L-alanine and L-anticapsin.As shown earlier by our research group, the synthesis of bacilysin is regulated by bacAoperon under the control of quorum sensing and s...
Bacillus subtilis ΔbacA mutantının karşılaştırmalı dinamik sekretom analizi
Özcengiz, Gülay (2017-11-24)
Sekretom, hücre dışına salgılanan proteinleri ve bu proteinlerin salgılanma mekanizmalarına ait elemanları içerir. Sekretom çalışmaları, birçok organizmanın değişen koşullar altında salgıladıkları belirli özelliklere sahip proteinlerin tanımlanıp kullanım alanları yaratmaya olanak sağlamada önemli konuma sahiptir. B. subtilis in belirli suşları tarafından üretilebilen basilisin L-alanin and L-antikapsin den oluşan dipeptid yapısında bir antibiyotiktir. Basilisin biyosentizinin hücre yoğunluğu sinyali mekani...
Response surface optimization of Bacillus thuringiensis israelensis fermentation
Tokçaer, Zeynep; Özcengiz, Gülay; Alaeddinoğlu, Gürdal; Department of Biology (2003)
The control of pest populations by using insect pathogens has been an attractive alternative to the application of chemical pesticides employed for the same purpose. As these chemicals not only damage the environment, but also trigger development of resistance by the pests and can harm other organisms together with the target pest, biological control is preferable and Bacillus thuringiensis (Bt) subspecies have been the most widely used bioinsecticides in forestry, agriculture and mosquito/ black fly contro...
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G. Özcengiz, “Bacillus thuringiensis alttür morrisoni Kitinaz Geninin Karakterizasyonu,” 2007, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/75273.