Klavulanik asit aşırı üreticisi endüstriyel S. clavuligerus suşunda fizyolojik değişimlerin proteomik yöntemler ile belirlenmesi



Integration of clavaminate synthase 2 gene into the chromosome of an industrial strain of Streptomyces Clavuligerus for enhanced clavulanic acid production
Vanlı, Güliz; Özcengiz, Gülay; Özkan, Melek; Department of Biotechnology (2010)
Streptomyces clavuligerus is a gram-positive, filamentous bacterium which has a great ability to produce secondary metabolites including isopenicillin N, cephamycin C and a beta-lactamase inhibitor clavulanic acid. Clavulanic acid (CA) which is a bicyclic beta-lactam, inhibits most of class A beta-lactamases by binding irreversibly to the serine hydroxyl group at the active center of beta-lactamases and resulting in the stable acyl-enzyme complexes. Clavaminate synthase (CAS) is one of the best characterize...
Reactive extraction of pyruvic acid from aqueous single and mixed acid solutions
Martı, Mustafa Esen; Gürkan, Türker; Department of Chemical Engineering (2010)
Interest in recovery of carboxylic acids from their aqueous solutions and production media has been growing with the improvements in the production techniques. Reactive extraction is proposed as a promising method to achieve high distribution coefficients with good selectivity. In the present study, equilibrium and kinetic studies, needed for the design of a recovery unit were carried out. Reactive extraction of pyruvic, acetic and lactic acids from their single and mixed acid solutions were carried out wit...
Small molecule ubiquitin-proteasome system inhibitor library screen reveals botulinum neurotoxin type a inhibitors
Şen, Edanur; Kiriş, Erkan; Department of Biology (2021-2-15)
Botulinum neurotoxins (BoNTs), known as the most potent bacterial toxins, cause potentially lethal disease botulism. BoNTs are classified as category A bioterror agents by many countries because they are easy to produce and have extreme toxicities. On the other hand, BoNTs are considered a therapeutic marvel in medicine because they have been extensively utilized not only for cosmetic purposes to get rid of wrinkles but also to treat various conditions in clinics, including movement disorders. These toxins ...
Pretreatment of cotton stalks with ionic liquids for enhanced enzymatic hydrolysis of cellulose and ethanol production
Haykır, Nazife Işık; Bölükbaşı, Ufuk; Department of Chemical Engineering (2013)
This study aims efficient conversion of cotton stalks to cellulosic ethanol through ionic liquid pretreatment and enhanced enzymatic hydrolysis. Among several ionic liquids, EMIMAc exhibited the most striking impact on cotton stalks with respect to the changes in biomass structure and digestibility. Cotton stalks, which were subjected to EMIMAc pretreatment at 10% (w cotton stalks/w EMIMAc) of biomass loading and 150°C for 30 minutes, were found to be 9 times more digestible than untreated cotton stalks. Be...
Genetic differentiation of European black poplar (Populus Nigra L.) clones and populations with respect to some enzymes involved in biosynthesis of cellulose and lignin
Taşkıran, Bircan; Kaya, Zeki; Department of Biology (2020)
The European black poplar, (Populus nigra L.) is ecologically and economically important fast-growing trees. Its wide distribution range in northern hemisphere, easy vegetative propagation, rapid growth rate and hybridization ability, and also its biomass source potential make European black poplar a perfect candidate for lignocellulosic biomass production. In this study, specific activities of two important cellulose related (SuSy and UGPase) and three lignin pathway related (PAL, 4CL, and CAD) enzymes, we...
Citation Formats
G. Özcengiz, “Klavulanik asit aşırı üreticisi endüstriyel S. clavuligerus suşunda fizyolojik değişimlerin proteomik yöntemler ile belirlenmesi,” 2017, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/80864.