Genetic differentiation of European black poplar (Populus Nigra L.) clones and populations with respect to some enzymes involved in biosynthesis of cellulose and lignin

Taşkıran, Bircan
The European black poplar, (Populus nigra L.) is ecologically and economically important fast-growing trees. Its wide distribution range in northern hemisphere, easy vegetative propagation, rapid growth rate and hybridization ability, and also its biomass source potential make European black poplar a perfect candidate for lignocellulosic biomass production. In this study, specific activities of two important cellulose related (SuSy and UGPase) and three lignin pathway related (PAL, 4CL, and CAD) enzymes, were evaluated in natural P. nigra clones. In addition to cellulose and lignin related enzyme activities, cellulose, lignin, and glucose contents, as well as height and diameter of clones were examined to explore genetic variance components, heritability of traits, and relationships between these traits. Also, clones with lignocellulosic potential were identified to be used for future breeding studies and industrial applications. As a total, 285 clones (genotypes) represented by 4 ramets and replicated twice are used for the study. The results of our study indicated that the great portion of total variation was due to clonal variation ranging from 33.0% (in UGPase) to 64.1% (in glucose). Moderate to high clonal heritabilities were estimated for traits ranging from 0.50 (in UGPase) to 0.80 (in glucose). All lignin related traits showed moderate heritabilities ranging from 0.53 (in CAD) to 0.61 (in PAL). Number of clones with desired cellulose and lignin properties were found. One of these clones, 62160 was among the highest 12 clones regarding UGPase, SuSy, cellulose, height, and diameter, but it was also among the lowest 12 clones with respect to PAL and CAD activities. Phenotypic correlations of cellulose content with SuSy (rp=0.184) and UGPase (rp=0.160) were low, but significant. Although PAL, 4CL, and CAD were positively correlated with each other, surprisingly there were no significant relationships detected between lignin content and these enzymes. The positive correlation among these phenylpropanoid pathway enzymes clearly demonstrated the flux of the substrate into the pathway, but no relationships between lignin content and them suggested that there might be more complicated regulations in the pathway which leads to change in lignin composition rather than lignin deposition.


Genetic differentiation between clone collections and natural populations of European black poplar (Populus nigra L.) in turkey
Çiftçi, Asiye; Kucukosmanoglu, Filiz; Karahan, Alptekin; Kaya, Zeki (2017-06-01)
The European black poplar (Populus nigra L.) is an ecologically and economically important tree species for Turkey. The important and major genetic resources of species for future breeding and ex situ conservation purposes have been archived in a clone bank in Ankara by selecting clones from natural populations and old plantations throughout Turkey. There is no study to date assessing genetic composition these materials. Two-hundred-thirty-three P. nigra clones from six geographic region of Turkey (clone co...
Genetic control of 4 coumarate coenzyme A ligase (4CL9 enzymes involved in lignin biosynthesis of European black poplar (Populus nigra L.)
Taşkıran, Bircan; Güray, Nülüfer Tülün; Kaya, Zeki (null; 2018-01-13)
European black poplar (Populus nigra L.) is considered as one of the most economically significant forest trees with respect to production of wood, pulp, bioenergy and other wood-based products. The content and quality of wood are greatly affected by plant cell wall compositions (cellulose, hemicellulose, and lignin). Although lignin serves as a mechanical barrier as well as protection against pests and pathogens, it emerges as an undesirable polymer for both pulp and bioenergy manufacturing industries beca...
Genetic diversity of european black poplar (populus nigra) populations from Turkey assessed by microsatellite DNA markers
Çiftçi, Asiye; Kaya, Zeki; Department of Biology (2013)
The genus Populus is known as a group of forest trees for commercial exploitation. The European black poplar (Populus nigra L.), belonging to the genus Populus, is a keystone species of floodplain forests in Europe. Populus nigra has also been used as a model tree in the study of genetic resource conservation of wild relatives of cultivated plants. Turkey has over 68.000 ha poplar plantations with P. nigra and 77. 000 ha consisting of various hybrids of it. Due to overexplotation, loss of natural distributi...
Genetic diversity and structure of Populus nigra populations in two highly fragmented river ecosystems from Turkey
Çiftçi, Asiye; Kaya, Zeki (Springer Science and Business Media LLC, 2019-08-01)
European black poplar (Populus nigra L.) is an important tree species in terms of social, economic, and ecological interest in Turkey. Although large poplar plantations meet the needs of the economy, natural genetic resources of the species have been highly degraded due to anthropogenic effects such as overexploitation and habitat fragmentations. To assess genetic diversity and structure of fragmented populations, 124 naturally distributed European black poplar trees from two major rivers (Kızılırmak and Gö...
Akkad, Raitha; Kaya, Zeki; Department of Molecular Biology and Genetics (2022-5-27)
Populus nigra L. owes to be the most critical pioneer tree species in riparian ecosystems and is considered an ecologically and economically-wise imperative fast-growing species. Moreover, it is a great tree species for biomass and bioenergy utilization and cellulose content manipulation. The limited energy resources from fossil fuels demand an alternative efficient biomass crop, where Populus nigra shows to be a great candidate. In this study, the CesA (cellulose synthase) secondary cell wall-related CesA4...
Citation Formats
B. Taşkıran, “Genetic differentiation of European black poplar (Populus Nigra L.) clones and populations with respect to some enzymes involved in biosynthesis of cellulose and lignin,” Thesis (Ph.D.) -- Graduate School of Natural and Applied Sciences. Biology., Middle East Technical University, 2020.