Thermal Study of Heavy Metal-Tolerant Gordonia, Acinetobacter and Microbacterium Species for Investigation of Bioremediation

2017-10-07
Gurbanov, Rafig
Gözen, Ayşe Gül
Severcan, Feride
The first step in bioremediation of heavy metal polluted soil/water is to select the heavy metaltolerant microbes from others. In this, major drawback is the insufficiency of the timeconsuming and expensive selection/characterization techniques, which necessitates the development of accurate and sensitive technology. In this study, we applied Differential Scanning Calorimetry (DSC) technique, to characterize heavy metal-tolerant bacteria at molecular level. For DSC experiments, we used bacteria derived from the surface mucus of freshwater fish and exposed them to toxic concentrations of cadmium (Cd), lead (Pb) and silver (Ag) and selected most tolerant ones. We found structural changes in biomolecules of tolerant bacteria compared to non-tolerant ones. Each bacteria showed unique peak profile at three different thermal regions which are assigned for ribosomal proteins, nucleic acids and cell wall components. Our findings evidenced shifts, diminishes or losses of the thermal peaks. We also measured unique thermal stability changes in ribosomes, nucleic acids and cell wall constituents corresponding to conformational alterations in molecular structure. Unique alterations proved that, DSC can easily differentiate and select the heavy metal tolerant-bacteria at high sensitivity and accuracy. Fascinatingly, the bacteria are not dying as a result of huge changes, instead continuing their lives in linearity with the degree of adaptation process and become tolerant to heavy metal environments. This ability of bacteria can be used to develop novel heavy metal bioremediation strategies for ecosystem restoration.

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Citation Formats
R. Gurbanov, A. G. Gözen, and F. Severcan, “Thermal Study of Heavy Metal-Tolerant Gordonia, Acinetobacter and Microbacterium Species for Investigation of Bioremediation,” presented at the VIII. International symposium on ecology and environmental problems ISEEP(2017), Çanakkale, Türkiye, 2017, Accessed: 00, 2021. [Online]. Available: iseep2017.comu.edu.tr.