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Biochemical and genetic characterization of halobacterium salinarium strain isolated from Tuz Lake in central Anatolia

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2004
Çakıcı, Özgür
In this study, a halophilic archaea Halobacterium salinarium TG13 which is isolated from Tuz Lake in Central Anatolia was characterized biochemically and genetically. Halobacterium salinarium DSM3754 and Halobacterium salinarium S9 strains were used as a reference strain through the experiments. In biochemical characterization; total protein profiles of strains was compared by using 1D SDS PAGE. Total protein profile of the isolated strain has shown differences. The SDS-PAGE profile of the purified purple membrane showed only single band by coomassie staining. Molecular weight and pI values of the protein isolated from Halobacterium salinarium TG13 and Halobacterium salinarium S9 were estimated by 2D SDS-PAGE as 22 kD and 5.4, respectively. Photoactivity of purple membrane of the strains was investigated. pH change of the purple membranes were observed upon illumination. This protein might be corresponded to bacteriorhodopsin. In genetical characterization; polymorphism of genomic DNA of strains was scanned with RAPD-PCR. Plasmid DNA profiles of strains was determined to make use of RFLP technique. RAPD-PCR and RFLP analyses have shown that Halobacterium salinarium TG13 is different strain from reference Halobacterium salinarium strains (H.s. S9 and H.s. DSM3754).