Biochemical and genetic characterization of halobacterium salinarium strain isolated from Tuz Lake in central Anatolia

Çakıcı, Özgür
In this study, a halophilic archaea Halobacterium salinarium TG13 which is isolated from Tuz Lake in Central Anatolia was characterized biochemically and genetically. Halobacterium salinarium DSM3754 and Halobacterium salinarium S9 strains were used as a reference strain through the experiments. In biochemical characterization; total protein profiles of strains was compared by using 1D SDS PAGE. Total protein profile of the isolated strain has shown differences. The SDS-PAGE profile of the purified purple membrane showed only single band by coomassie staining. Molecular weight and pI values of the protein isolated from Halobacterium salinarium TG13 and Halobacterium salinarium S9 were estimated by 2D SDS-PAGE as 22 kD and 5.4, respectively. Photoactivity of purple membrane of the strains was investigated. pH change of the purple membranes were observed upon illumination. This protein might be corresponded to bacteriorhodopsin. In genetical characterization; polymorphism of genomic DNA of strains was scanned with RAPD-PCR. Plasmid DNA profiles of strains was determined to make use of RFLP technique. RAPD-PCR and RFLP analyses have shown that Halobacterium salinarium TG13 is different strain from reference Halobacterium salinarium strains (H.s. S9 and H.s. DSM3754).


Characterization and purification of a bacteriocin produced by Leuconostoc mesenteroides subsp. cremoris
Dündar, Halil; Bozoğlu, Faruk; Department of Biotechnology (2006)
In this study, a new bacteriocin isolated from a Leuconostoc mesenteroides subsp. cremoris strain was purified to homogeneity by pH mediated cell adsorption-desorption, solid phase extraction with Amberlite XAD-16, cation-exchange chromatography and hydrophobic interaction chromatography. The purification resulted in an electrophoretically pure protein that was smaller than 6 kDa as judged by SDS-PAGE. The bacteriocin was found to be very hydrophobic and cationic and could be adsorbed by synthetic calcium s...
Charaterization and indentification of bacteriocins from two lactococcus lactis subs lactis strains
Akçelik, Oya; Özcengiz, Gülay; Department of Biotechnology (2004)
In this study, bacteriocins from two L. lactis subsp. lactis isolates of Turkey origin designated OC1 and OC2, respectively, were characterized and identified. The activity spectra of the bacteriocins were determined by using different indicator bacteria including Listeria, Bacillus and Staphylococcus spp. Bacteriocins were tested for their sensitivity to different enzymes, heat treatments and pH values. Loss of bacteriocin activities after ?-amylase treatment suggested that they form aggregates with carboh...
Multiple antibiotic resistance of surface mucus dwelling bacterial populations in freshwater fish
Özaktaş, Tuğba; Gözen, Ayşe Gül; Department of Biology (2007)
Surface mucus of a freshwater fish, Alburnus alburnus (bleak), caught from Lake Mogan, situated in south of Ankara, was collected in different seasons. The total cultivable bacteria were enumerated by spread plate method on nine different media. Bacteria were isolated based on colony morphologies and pigmentation. A total of sixty bacterial isolates obtained. The mucus-dwelling bacteria were first tested for resistance against ampicillin and kanamycin; then streptomycin and chloramphenicol were added to the...
Cloning and characterization of Streptomyces clavuligerus meso-diaminopimelate decarboxylase (lysA) gene
Yağcıoğlu, Çiğdem; Özcengiz, Gülay; Department of Biology (2004)
In Streptomyces clavuligerus, the route to the biosynthesis of a-aminoadipic acid (a-AAA) represents an important primary metabolic pathway providing carbon flux to the synthetases of antibiotic formation. This carbon flow comes through the lysine-specific branch of the aspartate pathway and is rate limiting in the formation of cephamycin C, a second generation cephalosporin produced by this organism. In this study, the lysA gene which encodes for an important key enzyme of aspartate pathway; meso-diaminopi...
Identification of serotype specific dna marker for salmonella typhimurium by rapd-pcr method
Aksoy, Ceren; Gültekin, Güzin Candan; Department of Biotechnology (2004)
This study was performed for the identification of specific DNA marker using RAPD-PCR (Random Amplified Polymorphic DNA) method for serotype Salmonella Typhimurium which is one of the most prevalent serotype causing food poisining all over the world. The Primer 3 (RAPD 9.1), 5̕-CGT GCA CGC-3ء, was used in RAPD-PCR with 35 different Salmonella isolates. 12 of them were serotype Salmonella Typhimurium and 23 of them were belonging to other six different serotypes. Accordingly, two different 300 bp and 700 bp ...
Citation Formats
Ö. Çakıcı, “Biochemical and genetic characterization of halobacterium salinarium strain isolated from Tuz Lake in central Anatolia,” M.S. - Master of Science, Middle East Technical University, 2004.