Quick Discrimination of Heavy Metal Resistant Bacterial Populations Using Infrared Spectroscopy Coupled with Chemometrics

Date

2015-10-06

Author

Gurbanov, Rafig
Ozek, Nihal Simsek
Gözen, Ayşe Gül
Severcan, Feride

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

232
views

0
downloads

Lead and cadmium are frequently encountered heavy metals in industrially polluted areas. Many heavy metal resistant bacterial strains have a high biosorption capacity and thus are good candidates for the removal of toxic metals from the environment. However, as of yet there is no accurate method for discrimination of highly adaptive bacterial strains among the populations present in a given habitat. In this study, we aimed to find distinguishing molecular features of lead and cadmium resistant bacteria using Attenuated Total Reflectance Fourier Transformed Infrared (ATR-FT-IR) spectroscopy and chemometric approaches. Our results demonstrated that both control and metal exposed E. coli and S. aureus strains could be successfully discriminated from each other using hierarchical cluster and principal component analysis methods. Moreover, we found that lead exposed bacterial strains could be successfully discriminated from cadmium exposed ones with a high heterogeneity value. These clear discriminations can be described by the ability of a bacterium to change its metabolism in terms of the content and structure of cellular macromolecules under heavy metal stress. In our case, cadmium and lead-induced genetic response systems in bacteria caused remarkable alterations in overall cellular metabolism. Bacteria deal with a heavy metal stress by altering nucleic acid methylations and lipid and protein synthesis. Heavy metal burden led to the development of relevant metabolic changes in proteins, lipids, and nucleic acids of the resistant bacteria described in this study. Our approach showed that infrared spectra obtained via ATR-FT-IR spectroscopy coupled with chemometric analysis can be utilized for rapid, low-cost, informative, reliable, and operator-independent discrimination of resistant bacterial populations.

Subject Keywords

Lipids, Cadmium, Bacteria, Genetics, Heavy metals

URI

https://hdl.handle.net/11511/39761

Journal

ANALYTICAL CHEMISTRY

DOI

https://doi.org/10.1021/acs.analchem.5b01659

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Development of fluorescent whole-cell bacterial bioreporters for detection of inorganic arsenic and cadmium Elçin, Evrim; Öktem, Hüseyin Avni; Department of Biotechnology (2019) Environmental heavy metal contamination in many regions of the world is a serious problem of ecological health. Fast and constant monitoring of their levels is significant for both preventing their accumulation and taking an immediate action for removal. As an alternative approach to standard laboratory techniques, various biosensor systems for detection of heavy metals have been proposed. Bacterial biosensors hold great promise for in-field detection of heavy metals. In this study, two different whole cell...
Development of a precipitation based separation scheme for selective removal and recovery of heavy metals from cadmium rich electroplating industry effluents Islamoglu, Sezin; Yılmaz, Levent; Ozbelge, H. O. (2006-01-01) The treatment of electroplating wastes is a serious worldwide problem, because of their high content of many different heavy metals. Chemical precipitation based treatment methods could be an important alternative for fractional selective separation of heavy metals if they are systematically developed by sequencing of pH, adjusting the added portions of precipitating agents, and selecting the optimum time period before removing the precipitate from the solution. In this study, for selective removal and reco...
Molecular characterization of acutely and gradually heavy metal acclimated aquatic bacteria by FTIR spectraoscopy Kepenek, Eda S.; Gözen, Ayşe Gül; Severcan, Feride (Wiley, 2019-05-01) In the environment, bacteria can be exposed to the concentration gradient of toxic heavy metals (gradual) or sudden high concentration of them (acute). In both situations, bacteria get acclimated to toxic heavy metal concentrations. Acclimation causes metabolic and molecular changes in bacteria. In this study, we aimed to understand whether there are differences between molecular profiles of the bacteria (Brevundimonas, Gordonia and Microbacterium) which are under acute or gradual exposure to cadmium or lea...
Comparison of trace metal concentrations in malign and benign human prostate Yaman, Mehmet; Atici, Demet; Bakirdere, Sezgin; Akdeniz, Ismail (American Chemical Society (ACS), 2005-01-27) Imbalance in the composition of trace metals, recognized to be essential to normal human homeostasis, besides the accumulation of potentially toxic or nonessential trace metals, may cause disease. Thus, there is a need for their analysis in cancerous and noncancerous human tissues to examine the relationship between cancer and these elements. Trace metal concentrations including Cd, Ni, Zn, Cu, Fe, Mg, and Ca in both malign and benign prostate samples were determined by atomic absorption spectrophotometry. ...
Use of clinoptilolite for copper and nickel removel from aqueous solutions Çağın, Volkan; İmamoğlu, İpek; Department of Environmental Engineering (2006) Heavy metals are well known toxic priority pollutants. Hence, wastewaters containing these species must be treated prior to discharge into receiving bodies. In this study, the potential of Bigadiç clinoptilolite for Cu2+ and Ni2+ removal from wastewaters was investigated in batch and continuous reactors. Results of the preliminary experiments revealed the optimum operating conditions, namely, initial solution pH of 5 and 4 for Cu2+ and Ni2+, respectively and contact time of 48 hours. Additionally, condition...

Citation Formats

R. Gurbanov, N. S. Ozek, A. G. Gözen, and F. Severcan, “Quick Discrimination of Heavy Metal Resistant Bacterial Populations Using Infrared Spectroscopy Coupled with Chemometrics,” ANALYTICAL CHEMISTRY, pp. 9653–9661, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/39761.