Biological hydrogen production from olive mill wastewater and its applications to bioremediation

Download

index.pdf

Date

2006

Author

Eroğlu, Ela

Metadata

Show full item record

Item Usage Stats

370
views

111
downloads

Hydrogen production by photosynthetic bacteria occurs under illumination in the presence of anaerobic atmosphere from the breakdown of organic substrates, which is known as photofermentation. In this study, single-stage and two-stage process development were investigated for photofermentative hydrogen production from olive mill wastewater by Rhodobacter sphaeroides O.U.001 within indoor and outdoor photobioreactors. It was proven that diluted olive mill wastewater (OMW) could be utilized for photobiological hydrogen production as a sole substrate source. However, pretreatment of the system is needed to reduce the dark color and bacteriostatic effects of OMW. In this study, several two stage processes including pretreatment of OMW followed by photofermentation were investigated to increase the hydrogen production yields in addition to the significant remediation of OMW. Explored pretreatment methods contain chemical oxidation with ozone or Fenton’s reagent, photodegradation by UV radiation, adsorption with clay or zeolite and dark fermentation with acclimated or non-acclimated sewage sludge. Among these different two-stage processes; clay treatment method resulted the highest hydrogen production capacity. As a result of clay pretreatment, 65% of the initial color and 81% of the phenolic content were decreased. Hydrogen production capacity was 16 LH2/LOMW without pretreatment, while it was enhanced up to 29 LH2/LOMW by two-stage processes. Moreover, clay pretreatment process made it possible to utilize highly concentrated OMW (50% and 100%) media for hydrogen production and for remediation. On the aspects of environment, treatment of OMW was achieved in the present work. The final composition of the organic pollutants in the effluent of two-stage processes was below the wastewater discharge limits. The overall results obtained throughout this study may open a new opportunity for the olive oil industry and for the biohydrogen area as a result of the effective biotransformation of OMW into hydrogen gas and valuable by-products.

Subject Keywords

Biotechnology., Hydrogen.

URI

http://etd.lib.metu.edu.tr/upload/3/12607264/index.pdf
https://hdl.handle.net/11511/16520

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Kinetic analysis of photosynthetic growth, hydrogen production and dual substrate utilization by Rhodobacter capsulatus Sevinc, Pelin; Gündüz, Ufuk; EROĞLU, İNCİ; Yucel, Meral (2012-11-01) Rhodobacter capsulatus is purple non-sulfur (PNS) bacterium which can produce hydrogen and CO2 by utilizing volatile organic acids in presence of light under anaerobic conditions. Photofermentation by PNS bacteria is strongly affected by temperature and light intensity. In the present study we present the kinetic analysis of growth, hydrogen production, and dual consumption of acetic acid and lactic acid at different temperatures (20, 30 and 38 degrees C) and light intensities (1500, 2000, 3000, 4000 and 50...
Phototrophic hydrogen production by agar-immobilized Rhodobacter capsulatus Elkahlout, Kamal E. M.; Yücel, Ayşe Meral; Eroğlu, İnci; Department of Biotechnology (2011) photosynthetic bacteria is attractive field as production is fueled by solar energy. Hydrogen production potential of two photosynthetic bacteria R.capsulatus (DSM1710 wild type and R.capsulatus YO3 Hup- uptake hydrogenase deleted mutant strain) were examined in agar immobilized systems. In the present work agar and glutamate concentrations were optimized for immobilization of bacteria while feeding bacteria with 40/2-4 mM acetate/ glutamate. Immobilized bacteria produced hydrogen for 420-1428 hours coverin...
Investigation of sugar metabolism in rhizopus oryzae Büyükkileci, Ali Oğuz; Hamamcı, Haluk; Department of Biotechnology (2007) Rhizopus oryzae is a filamentous fungus, which can produce high amounts of L(+)-lactic acid and produces ethanol as the main by-product. In an effort to understand the pyruvate branch point of this organism, fermentations under different inoculum and glucose concentrations were carried out. At low inoculum size (1x103 spores ml-1), high amount of lactate (78 g l-1) was produced, whereas high ethanol concentration (37 g l-1) was obtained at high inoculum sizes (1x106 spores ml-1). Decreasing working volume i...
Fe-catalyzed sulfide oxidation in hydrothermal plumes is a source of reactive oxygen species to the ocean Shaw, Timothy J.; Luther, George W.; Rosas, Richard; Oldham, Veronique E.; Coffey, Nicole R.; Ferry, John L.; Dias, Dewamunnage M. C.; Yücel, Mustafa; de Chanvalon, Aubin Thibault (2021-10-01) Historically, the production of reactive oxygen species (ROS) in the ocean has been attributed to photochemical and biochemical reactions. However, hydrothermal vents emit globally significant inventories of reduced Fe and S species that should react rapidly with oxygen in bottom water and serve as a heretofore unmeasured source of ROS. Here, we show that the Fe-catalyzed oxidation of reduced sulfur species in hydrothermal vent plumes in the deep oceans supported the abiotic formation of ROS at concentratio...
Direct synthesis of hydrogen storage alloys from their oxides Tan, Serdar; Öztürk, Tayfur; Aydınol, Mehmet Kadri; Department of Metallurgical and Materials Engineering (2011) The aim of this study is the synthesis of hydrogen storage compounds by electrodeoxidation technique which offers an inexpensive and rapid route to synthesize compounds from oxide mixtures. Within the scope of this study, two hydrogen storage compounds, FeTi and Mg2Ni, are aimed to be produced by this technique. In the first part, effect of sintering conditions on synthesis of FeTi was studied. For this purpose, oxide pellets made out of Fe2O3-TiO2 powders were sintered at temperatures between 900 °C – 1300...

Citation Formats

E. Eroğlu, “Biological hydrogen production from olive mill wastewater and its applications to bioremediation,” Ph.D. - Doctoral Program, Middle East Technical University, 2006.