Expressions and functions of phosphodiesterase enzymes are different in different region of the rat heart

Date

2016-06-30

Author

Derici, Mehmet Kursat
Sadi, Gökhan
Cenik, Başar
Güray, Nülüfer Tülün
Demirel Yılmaz, Emine

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Phosphodiesterase (PDE) enzymes are responsible for the breakdown of cAMP and cGMP and adjust the levels of cyclic nucleotides in heart. Alteration of PDE enzymes expression in different tissues caused conflicts between functional and clinical effects of PDE inhibitors. Therefore the aim of this study was to investigate the functional role and gene/protein expression of PDE enzymes in atrial and ventricular regions of the rat heart. The spontenously beating right atrium and electrically stimulated left papillary muscles from Wistar albino rats were placed in organ bath. The effects of PDE1, 2, 3, 4, 5 inhibitors (Vinpocetine, EHNA, Milrinone, Rolipram Sildenafil) on functions were recorded. The expressions of PDE’s in tissues as well as in enzymatically isolated atrial and ventricular cells were examined by Real Time PCR and Western blot. Right atrial contractions have been increased with Vinpocetine while suppressed by EHNA, Milrinone, Rolipram and Sildenafil. Unlike Milrinone and Sildenafil, Vinpocetine increases the heart rate whereas EHNA and Rolipram have not any significant effect. Left papillary muscle contractions have been increased only with the Vinpocetine while other PDE inhibitors have not been the emergence of a significant effect. The mRNA and protein levels of PDE enzymes were significantly different in atrial and ventricular intact tissues or isolated cells. PDE-1, 2, 3, 4 and 5 enzymes show functional and regional differences in the rat heart. Therefore, these differences should be taken into account in the experimental or therapeutic approaches of the heart.

Subject Keywords

Phosphodiesterases, Right atrium, Papillary muscle

URI

https://hdl.handle.net/11511/87220
https://www.ephar2016.org/post_congress_book.pdf

Conference Name

7th European Congress of Pharmacology (EPHAR2016), 26-30 June 2016

Collections

Department of Biology, Conference / Seminar

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Citation Formats

M. K. Derici, G. Sadi, B. Cenik, N. T. Güray, and E. Demirel Yılmaz, “Expressions and functions of phosphodiesterase enzymes are different in different region of the rat heart,” presented at the 7th European Congress of Pharmacology (EPHAR2016), 26-30 June 2016, Istanbul, TURKEY, 2016, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/87220.