Stability of a recombinant plasmid carrying alpha-amylase gene in Bacillus subtilis

1998-03-01
Plasmid pSB6 is a streptococcal recombinant plasmid carrying the alpha-amylase gene of Bacillus amyloliquefaciens and the chloramphenicol resistance gene. The segregational and structural instabilities of this plasmid were examined under non-selective conditions in Bacillus subtilis. These instabilities were modelled according to a kinetic expression derived from the difference in the growth between plasmid-bearing and plasmid-free cells. This plasmid showed slight segregational instability and much higher levels of structural instability under the conditions examined.

Citation Formats
G. C. Gültekin, A. Bayındırlı, Z. Ogel, and P. Tanboga, “Stability of a recombinant plasmid carrying alpha-amylase gene in Bacillus subtilis,” WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol. 14, no. 2, pp. 293–295, 1998, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46917.