Stability of a recombinant plasmid carrying alpha-amylase gene in Bacillus subtilis

Date

1998-03-01

Author

Gültekin, Güzin Candan
Bayındırlı, Alev

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Plasmid pSB6 is a streptococcal recombinant plasmid carrying the alpha-amylase gene of Bacillus amyloliquefaciens and the chloramphenicol resistance gene. The segregational and structural instabilities of this plasmid were examined under non-selective conditions in Bacillus subtilis. These instabilities were modelled according to a kinetic expression derived from the difference in the growth between plasmid-bearing and plasmid-free cells. This plasmid showed slight segregational instability and much higher levels of structural instability under the conditions examined.

Subject Keywords

Alpha-amylase, Bacillus subtilis, Plasmid instability

URI

https://hdl.handle.net/11511/46917

Journal

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

DOI

https://doi.org/10.1023/a:1008815120601

Collections

Department of Food Engineering, Article

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Citation Formats

G. C. Gültekin and A. Bayındırlı, “Stability of a recombinant plasmid carrying alpha-amylase gene in Bacillus subtilis,” WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, pp. 293–295, 1998, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46917.