Immobilization of urease in various polymeric matrices

Demircioğlu, Hüsniye


Immobilization of glucoamylase onto activated pHEMA/EGDMA microspheres: properties and application to a packed-bed reactor
Arica, MY; Alaeddinoglu, NG; Hasırcı, Vasıf Nejat (1998-02-15)
Glucoamylase was covalently immobilized onto pHEMA/EGDMA microspheres of two different sizes: 50-100 mu m and 100-200 mu m in diameter. The activity of the enzyme on smaller microspheres was found to be almost twice that of the larger microspheres. A higher enzyme lending was observed on small microspheres (0.64 mg g(-1) support) as compared to large spheres (0.40 mg g(-1) support). The K-m of glucoamylase was significantly increased (approximately five times) upon immobilization, indicating decreased affin...
Immobilization of glucose isomerase in surface-modified alginate gel beads
In this study, glucose isomerase enzyme was entrapped into modified and nonmodified calcium alginate gel beads. Various characteristics of free and immobilized enzymes such as the optimum pH, temperature and dependence of activity on storage and operational stability were evaluated. The optimum pH and temperature of free and immobilized glucose isomerase were found to be the same values as 7.5 and 60C, respectively. For free and immobilized enzymes, kinetic parameters were calculated as 1.79 x 10(-2) and 8....
Inactivation of bovine liver arginase by l-ethl-3(3-dimethylaminopropy) carbodiimide
Türkoğlu, E Suna; Özer, İnci; Department of Biochemistry (1989)
Removal of cobalt from zinc sulfate solution by cementation prior to zinc electrowinning
Kayın, Pınar Burcu; Topkaya, Yavuz Ali; Department of Metallurgical and Materials Engineering (2003)
The aim of this study was to investigate the removal of cobalt from zinc sulfate solution by cementation with the help of conventional and new type of additives that were 4% Sn-zinc alloy powder and 10% Sn-zinc alloy powder, respectively. Synthetic leach solutions containing 150 g/l Zn and 75 mg/l Co were prepared and used in all of the experiments. The parameters researched with the conventional method were the amount of arsenic trioxide and the effect of copper sulfate on cementation of cobalt. While usin...
Enantioselective chemoenzymatic synthesis of oseltamivir (tamiflu)(r) intermediates
Eşiyok, Hacı; Demir, Ayhan Sıtkı; Department of Biotechnology (2008)
The objective of this presented study was to synthesize optically active compounds considered to be key intermediates in the synthesis of Oseltamivir (Tamiflu) by performing chemical and biotechnological methods. Thereof, the carboethoxy cyclohexenone skeleton first was synthesized utilizing easily available substances. The synthesis of alpha-hydroxy ketones in enantiomerically pure form offers a great importance in the synthesis of biologically active compounds. Toward this fact, the enantioselective synth...
Citation Formats
H. Demircioğlu, “Immobilization of urease in various polymeric matrices,” Ph.D. - Doctoral Program, Middle East Technical University, 1993.