Overproduction of neutral protease in Bacillus subtilis

Günal, Aysu


Overexpression of a serine alkaline protease gene in Bacillus licheniformis and its impact on the metabolic reaction network
Çalık, Pınar; Oliver, SG; Ozdamar, TH (Elsevier BV, 2003-05-20)
This work reports on cloning of serine alkaline protease (SAP) encoding gene subC to a multi-copy plasmid and its expression in Bacillus licheniformis with the quantitative impact of overexpression of the subC gene on metabolic flux distributions. Bioprocess characteristics of the wild-type and the recombinant B. licheniformis were investigated in a defined simple synthetic medium with glucose as the sole carbon source under well-defined bioreactor-operation conditions. Significant physiological changes wer...
Overexpression of serine alkaline protease encoding gene in Bacillus species: performance analyses
Çalık, Pınar; Ozdamar, TH (Elsevier BV, 2003-12-02)
Bacillus species carrying subC gene encoding serine alkaline protease (SAP) enzyme were developed in order to increase the yield and selectivity in the bioprocess for SAP production. For this aim, subC gene was cloned into pHV1431 Escherichia coli-Bacillus shuttle vector, and transferred into nine host Bacillus species, i.e. B. alvei, B. amyloliquefaciens, B. badius, B. cereus, B. coagulans, B. firmus, B. licheniformis, B. sphaericus and B. subtilis. The influence of the host Bacillus species on SAP product...
Differential expression of archaeal Hsp60 and CDC48 homologue VAT genes in heat shock
Kocabıyık, Semra; Demirok, B. (2007-07-01)
Differential alteration of IL-8 in liver cancer stem cell enrichment in response to PI3K/Akt/mTOR inhibitors and sorafenib
Kahraman, Deniz Cansen; Kahraman, Tamer; Atalay, Rengül (2018-07-01)
Liver cancer stem cells (LCSCs) are derived from damaged and transformed hepatic progenitor cells (HPCs) during precancerous cirrhosis stage. Ras/Raf/MAPK and PI3K/AKT/mTOR signaling pathways are significantly deregulated in liver cancer. The activation of PI3K/AKT/mTOR pathway in LCSC population is one of the reasons for acquired resistance to sorafenib in advanced hepatocellular carcinoma (HCC) patients. Therefore, identifying novel inhibitors targeting this pathway acting on LCSCs is highly essential. We...
Modulation of mRNA expression and activities of xenobiotic metabolizing enzymes, CYP1A1, CYP1A2, CYP2E1, GPx and GSTP1 by the Salicornia freitagii extract in HT-29 human colon cancer cells
ALTAY, AHMET; İrtem Kartal, Deniz; SADİ, GÖKHAN; Güray, Nülüfer Tülün; YAPRAK, AHMET EMRE (2017-01-01)
Phase I-II detoxification and antioxidant enzymes are responsible for the detoxification and elimination of activated carcinogens, acting as important biomarkers for chemoprevention. Among them, cytochrome P450s plays a prominent role in the metabolic activation of xenobiotics. The herb Salicornia freitagii (SF) (Amaranthaceae) is known for its anticancer, antioxidant, antidiabetic and antiinflammatory activities. In this study, we determined the bioactive phenolics in the SF methanol extract and investigat...
Citation Formats
A. Günal, “Overproduction of neutral protease in Bacillus subtilis,” Middle East Technical University, 1997.