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Overproduction of neutral protease in Bacillus subtilis
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068683.pdf
Date
1997
Author
Günal, Aysu
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https://hdl.handle.net/11511/8887
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Graduate School of Natural and Applied Sciences, Thesis
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This work reports on cloning of serine alkaline protease (SAP) encoding gene subC to a multi-copy plasmid and its expression in Bacillus licheniformis with the quantitative impact of overexpression of the subC gene on metabolic flux distributions. Bioprocess characteristics of the wild-type and the recombinant B. licheniformis were investigated in a defined simple synthetic medium with glucose as the sole carbon source under well-defined bioreactor-operation conditions. Significant physiological changes wer...
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A. Günal, “Overproduction of neutral protease in Bacillus subtilis,” Middle East Technical University, 1997.